舒筋活血片HPLC指纹图谱及多指标含量测定研究  

HPLC Fingerprint and Multicomponents Determination of Shujin Huoxue Tablets

作　　者：王林 Wang Lin(Affiliated Hangzhou First People's Hospital,Zhejiang University School of Medicine,Department of Pharmacy,Hangzhou 310006,China;Affiliated Hangzhou First People's Hospital,Zhejiang University School of Medicine,Key Laboratory of Clinical Cancer Pharmacology and Toxicology Research of Zhejiang Province,Hangzhou 310006,China)

机构地区：[1]浙江大学医学院附属杭州市第一人民医院药学部,杭州310006 [2]浙江大学医学院附属杭州市第一人民医院浙江省临床肿瘤药理与毒理学研究重点实验室,杭州310006

出　　处：《中国药师》2020年第7期1326-1331,共6页China Pharmacist

基　　金：浙江省医学重点学科资助《浙卫办[2018]2号》;浙江省临床肿瘤药理与毒理学研究重点实验室资助(编号:2020E10021)。

摘　　要：目的:建立舒筋活血片的HPLC指纹图谱及多指标成分测定方法。方法:采用HPLC法,以Agilent ZORBAX Eclipse XDB-C18柱(250 mm×4.6 mm,5μm)为色谱柱,以甲醇(A)-0.1%磷酸水溶液(B)为流动相,梯度洗脱,流速为0.8 ml·min^-1,检测波长为285 nm,柱温为30℃,进样量为20μl。以4-甲氧基水杨醛为参照,绘制13批样品的HPLC指纹图谱;采用《中药色谱指纹图谱相似度评价系统》(2004年A版)进行相似度评价,确定共有峰;采用SPSS 22.0软件对13批样品进行聚类分析,同时测定原儿茶酸、原儿茶醛、紫丁香苷、绿原酸、羟基红花黄色素A、芦丁、4-甲氧基水杨醛、α-香附酮、山柰素9种成分的含量。结果:13批舒筋活血片指纹图谱标定了共有峰24个,指认了其中9个化学成分。原儿茶酸、原儿茶醛、紫丁香苷、绿原酸、羟基红花黄色素A、芦丁、4-甲氧基水杨醛、α-香附酮、山柰素9种成分线性关系良好(r=0.9992~0.9998),平均回收率在97.8%~100.8%之间。结论:所建立的HPLC指纹图谱及多指标成分测定可为舒筋活血片的质量评价提供参考。Objective:To explore the HPLC fingerprint and multicomponents determination of Shujin Huoxue tablets.Methods:An HPLC method was adopted.The separation was performed on an Agilent ZORBAX Eclipse XDB-C18 column(250 mm×4.6 mm,5μm)with gradient elution by methanol(A)-0.1%phosphoric acid solution(B)at the flow rate of 0.8 ml·min^-1 and the detection wavelength of 285 nm.The column temperature was 30℃and the sample size was 20μl.Using 4-methoxysalicylaldehyde as the reference,the HPLC fingerprints of 13 batches of samples were determined.The similarity of the 13 batches of samples was evaluated by TCM Chromatographic Fingerprint Similarity Evaluation System(2004 A edition)to confirm the common peaks.Cluster analysis was performed by using SPSS 22.0 software.Meanwhile,nine components including protocatechuic acid,protocatechuic aldehyde,syringin,chlorogenic acid,hydroxysafflor yellow A,rutin,4-methoxysalicylaldehyde,α-cyperone and kaempferol were determined.Results:There were 24 common peaks in the fingerprints of the 13 batches of Shujin Huoxue tablets,and nine of which were identified.The contents of protocatechuic acid,protocatechuic aldehyde,syringin,chlorogenic acid,hydroxysafflor yellow A,rutin,4-methoxysalicylaldehyde,α-cyperone and kaempferol showed good linear relationships(r=0.9992-0.9998),and the average recovery was within the range of 97.8%-100.8%.Conclusion:The established HPLC fingerprint and multicomponent determination can provide reference for the quality evaluation of Shujin Huoxue tablets.

关 键 词：舒筋活血片 指纹图谱 原儿茶酸 原儿茶醛 紫丁香苷 绿原酸 羟基红花黄色素A 芦丁 4-甲氧基水杨醛 Α-香附酮 山柰素 

分 类 号：TQ460.72[医药卫生—药物分析学]