15位点重复单元-可变数目串联重复序列技术在河南地区结核分枝杆菌的基因分辨率的对比分析研究  

Comparison and analysis of gene resolution on Mycobacterium tuberculosis in Henan by 15-locus interspersed repeating unit-a variable number of tandem repeats

作　　者：张国龙 ZHANG Guo-long(Henan Provincial Center for Disease Control and Prevention,Zhengzhou,Henan 450016,China)

机构地区：[1]河南省疾病预防控制中心,河南郑州450016

出　　处：《中国卫生检验杂志》2020年第13期1551-1555,共5页Chinese Journal of Health Laboratory Technology

摘　　要：目的比较15位点分枝杆菌散在重复单元-可变数目串联重复序列(MIRU-VNTR)对河南省结核分枝杆菌的分辨力与其他地区的区别。方法使用Spoligotyping和MIRU-VNTR对菌株进行分型,分析北京家族基因型在性别、年龄和地区间的分布情况。将15个位点对本省菌株的分辨率与其他地区结果相比较。结果本省东部地区患者感染北京家族结核的比例高于其他地区,尤其高于西部地区。本省55岁以上的人群更易感染北京型菌株。7个位点(Mtub04、MIRU10、MIRU39、MIRU31、Mtub21、MIRU26和Qub26)对河南菌株的分辨能力优于其他地区。15位点MIRU-VNTR结合Spoligotyping的分辨力高于我国其他大部分地区,但略低于上海和黑龙江。结论北京基因型在本省的分布存在地区和年龄的差异。15个位点MIRU-VNTR的分辨力在本省和其他地区间存在明显不同,因此需针对本省菌株指定合适的分型方案。Objective To compare the gene resolution on Mycobacterium tuberculosis between Henan and other regions by 15-loci interspersed repetitive units-a variable number of tandem repeats(IRU-VNTR).Methods The strains were genotyped by Spoligotyping and IRU-VNTR typing.We investigated the distribution of the Beijing family genotypes in ages,regions and gender,and compared discriminatory power of 15-loci MIRU-VNTR between our data and published MIRU-VNTR profiles of TB in other areas.Results It is found that substantially larger proportions of tuberculosis patients were infected with Beijing family genotypes in the eastern regions than that in other regions of Henan,especially in the western region.The proportion of Beijing genotype isolates was higher in patients over 55 years old than other age groups in Henan.Seven loci(Mtub04,MIRU10,MIRU39,MIRU31,Mtub21,MIRU26 and Qub26)could more effectively discriminate MTB strains in Henan than other regions.The discrimination of 15-locus combined with Spoligotyping is higher in Henan than most of other regions,but slightly lower than Shanghai and Heilongjiang.Conclusion The distribution of Beijing genotype showed geographic and age diversity.The discriminatory power of 15-locus is obviously different between our province and other regions,so it is necessary to establish a suitable genotyping scheme for our region strains.

关 键 词：结核分枝杆菌 15位点重复单元-可变数目串联重复序列 河南省 比较性研究 

分 类 号：R378.91[医药卫生—病原生物学]