复方中草药对白点鲑和亚东鲑抗氧化、非特异性免疫及消化酶指标的影响  

Effects of Compound Chinese Herbal Medicine on Antioxidant,Non-specific Immunity and Digestive Enzyme Activity in Salvelinus pluvius and Salmo trutta fario

作　　者：孙学亮 杨树元 陈书奇 孟凡亮 陈成勋 郭永军 于宏 SUN Xueliang;YANG Shuyuan;CHEN Shuqi;MENG Fanliang;CHEN Chengxun;GUO Yongjun;YU Hong(Tianjin Key Laboratory of Aqua-ecology and Aquaculture,Department of Fishery Sciences,Tianjin Agricultural University,Tianjin 300384,China;Yangliuqing Agriculture Service Center in Xiqing Area of Tianjin City,Tianjin 300380,China;Fengpei Aquatic Products Co.,Ltd.,Tianjin 300450,China)

机构地区：[1]天津农学院水产学院,天津市水产生态及养殖重点实验室,天津300384 [2]天津市西青区杨柳青镇农业服务中心,天津300380 [3]天津丰沛水产养殖有限公司,天津300000

出　　处：《水产学杂志》2020年第3期7-12,共6页Chinese Journal of Fisheries

基　　金：天津市水产现代农业产业技术体系创新团队(ITTFRS2017014);天津市科技计划项目(18JCTPJC65100、18ZXYENC00110、14ZCZDSY00053);天津市高等学校“创新团队培养计划”现代水产生态健康养殖(TD13-5089).

摘　　要：在水温10~12℃下,将体质量(224.14±26.73)g的白点鲑Salvelinus pluvius和体质量(152.12±7.46)g的亚东鲑Salmo trutta fario饲养在直径1m、水深0.7m的玻璃钢材质圆桶中,在基础饲料中添加0.5%的复方中草药(大黄、黄芪、连翅、黄苓和丹皮)进行投喂,在投喂0d、7d、14d、21d和28d时从鱼尾柄腹侧用肝素处理的容器抽血采样,测定血液中总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、酸性磷酸酶(ACP)、碱性磷酸酶(AKP)、溶菌酶(LZM)、肠道淀粉酶(AMS)、脂肪酶(LPS)和蛋白酶活性,及丙二醛(MDA)和一氧化氮(NO)含量,研究复方中草药对鱼血液抗氧化能力、非特异性免疫力及消化酶活力的影响。结果表明:摄食含复方中草药饲料14d时的亚东鲑血液T-AOC活力和SOD活力显著高于0d(P<0.05),MDA含量和CAT活力各组间差异不显著(P>0.05);7d和14d的亚东鲑血液AKP活力和ACP活力显著高于其他组(P<0.05),14d时NO浓度和LZM活性与0d差异显著(P<0.05);肠道蛋白酶活力差异不显著(P>0.05),淀粉酶活力和脂肪酶活力在14d时在显著高于其他组(P<0.05)。摄食含复方中草药饲料14d的白点鲑血液T-AOC活力显著高于其他组(P<0.05),MDA含量和SOD活力差异不显著(P>0.05),21d时CAT活力显著高于其他组(P<0.05);AKP活力差异不显著(P>0.05),7d、14d时ACP活力和NO浓度显著高于其他组(P<0.05),21d时LZM含量显著高于其他组(P<0.05);14 d时淀粉酶活力显著高于其他组(P<0.05),肠道蛋白酶活力和脂肪酶活力差异不显著(P>0.05)。综上所述,摄食含复方中草药的饲料,对白点鲑、亚东鲑血液的抗氧化性、非特异性及消化酶指标有促进作用,投喂14 d时的效果最佳。At a water temperature of 10~12℃,the body mass(224.14±26.73)g of Salceinus pluvius and the body mass(152.12±7.46)g of Salmo trutta fario were raised in a FRP diameter drum with a diameter of 1m and a water depth of 0.7 m.Feeding a basal feed supplemented with 0.5%compound Chinese herbal medicine(rhubarb,scutellaria,wing,scutellaria and paeonol),When feeding 0 d,7 d,14 d,21 d and 28 d,the ventral side of the fish stalk was sampled with a heparin-treated container to measure total antioxidant capacity(T-AOC),superoxide dismutase(SOD),catalase(CAT),acid phosphatase(ACP)in the blood,alkaline phosphatase(AKP),lysozyme(LZM),intestinal amylase(AMS),lipase(LPS)and protease activity,and malondialdehyde(MDA)and nitric oxide(NO)levels,study the effect of compound Chinese herbal medicine on blood antioxidant capacity,non-specific immunity and digestive enzyme activity of fish.The results showed that the blood T-AOC activity and SOD activity of Salmo trutta fario after eating containing compound Chinese herbal medicine for 14 days were significantly higher than 0 d(P<0.05),and the difference between MDA content and CAT activity was not significant(P>0.05).The AKP activity and ACP activity of Salmo trutta fario in 7 d and 14 d were significantly higher than those in other time periods(P<0.05).At 14 d,NO concentration and LZMactivity were significantly different from0 d(P<0.05);There was no significant difference in intestinal protease activity(P>0.05).Amylase activity and lipase activity were significantly higher than other time periods at 14 d(P<0.05).Amylase activity and lipase activity were significantly higher than other time periods at 14 d(P<0.05).The T-AOC activity of Salvelinus pluvius after eating containing compound Chinese herbal medicine for 14 d was significantly higher than that of other time periods(P<0.05).The difference of MDA content and SOD activity was not significant(P>0.05).At 21 d,CAT activity was significantly higher than other periods(P<0.05).AKP activity was not significantly different(P>0.05),the

关 键 词：复方中草药 白点鲑 亚东鲑 抗氧化 非特异性免疫 消化酶 

分 类 号：S948[农业科学—水产养殖]