小麦成熟期产量及钾效率相关性状的全基因组关联分析  

Genome-wide association analysis for yield and potassium efficiency related traits of wheat at maturity stage

作　　者：徐易如 赵艳艳[1] 孙福来 郭营[1] 赵岩[1] 李斯深[1] 孔凡美[1] XU Yi-ru;ZHAO Yan-yan;SUN Fu-lai;GUO Ying;ZHAO Yan;LI Si-shen;KONG Fan-mei(State Key Laboratory of Crop Biology/National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources/Shandong Agricultural University,Tai’an,Shandong 271018,China;Binzhou Agriculture and Rural Affairs Bureau,Binzhou,Shandong 256600,China)

机构地区：[1]作物生物学国家重点实验室/土肥资源高效利用国家工程实验室/山东农业大学,山东泰安271018 [2]滨州市农业农村局,山东滨州256600

出　　处：《植物营养与肥料学报》2020年第6期1081-1090,共10页Journal of Plant Nutrition and Fertilizers

基　　金：“十三五”国家重点研发计划“七大作物育种”重点专项(2017YFD0100600);山东省现代农业产业技术体系。

摘　　要：【目的】探明不同钾供应条件下控制产量及钾效率相关性状的稳定的显著关联分子标记位点,为小麦产量及钾效率相关性状的遗传控制机理研究及相关基因的克隆提供参考。【方法】利用134个小麦品种(系)组成的群体为试验材料,设置正常供钾(T1)和不施钾(T2)两个处理,进行了2年田间试验(E1、E2)。对小麦成熟期株高、穗长、穗粒数及钾吸收、利用效率等23个性状进行了表型鉴定,分别定义了同年同一处理和同一处理两年平均共6个环境平均值。采用GLM+Q一般线性模型和MLM+K+Q混合线性模型相结合的方法,利用群体差异SNP分子标记(90K SNP芯片)对小麦产量和钾效率相关性状进行全基因组关联分析。【结果】与正常钾处理相比,不施钾处理条件下籽粒钾利用效率显著升高,单株钾累积量、单株钾含量及总小穗数等性状显著降低。供试小麦各性状的群体变异系数为6.98%~350.38%,有14个性状的遗传力在50%以上,以株高的遗传力最高(92.03%)。利用保留的7485个多态性好的群体差异(SNP)进行了全基因组关联分析,共检测到1420个分子标记位点与供试23个性状在P≤0.001水平存在显著关联,分布在21条染色体上。有1097个(77.25%)分子标记位点仅在一个关联分析环境中被检测到;能在至少两个关联分析环境中被检测到的相对稳定分子标记位点有323个,其中113个位点与钾效率相关性状有关,Tdurumcontig26281139、Kukric3072053等分子标记位点可以提高钾吸收效率,Exc19038571、BS0003914851等分子标记位点能够提高钾利用效率。在至少4个关联分析环境中被检测到的位点有22个,分别与株高、千粒重、穗粒数等5个性状相关。与株高和千粒重显著关联的分子标记位点RFLContig40692628和BS0000363251可同时在全部6个关联分析环境中检测到,平均贡献率为9.59%和13.66%,环境稳定性非常好,与株高的降低和千粒重的提高显著�【Objectives】Crops are found response genetically to potassium(K)nutrition,so the identification and localization of the molecular markers,that are stable and significantly correlated to traits of yield and K efficiency under different K nutrition levels,will provide reference for further genetic modification of related traits and genes cloning in breeding.【Methods】A group of 134 wheat varieties(or lines)were used in a two-years field experiment,in which applying K(T1)and not applying K(T2)were setup.The yield and K efficiency related traits of wheat were measured,and the average data of traits were calculated under 6 defined environments,as the respective average values of each treatment in one year and two years.Genome-wide association analysis was carried out for the tested traits using SNP molecular markers.【Results】Compared with K application treatment,the K use efficiencies were significantly higher,while the K contents and accumulation of individual plant and the total spikelet number were significantly lower under no K application treatment.The coefficients of variation of the traits were 6.98%–350.38%among the group of cultivars(lines),and 14 of traits had heritability of>50%,with the highest trait of plant height(92.03%).According to genome-wide association analysis on the 7485 SNPs with satisfactory diversity,a total of 1420 molecular marker sites were identified associating with 23 traits significantly(P≤0.001),and located in 21 chromosomes.There were 1097(77.25%)molecular marker sites detected in only one environment,and 323 molecular marker sites that could be stably identified in at least 2 environments,and 113 of them were related to K efficiency,e.g.the molecular marker sites Tdurumcontig26281139 and Kukric3072053 could upregulate the absorption of K,Exc19038571 and BS0003914851 could increase K use efficiency.There were 22 molecular marker sites that could be identified in at least 4 environments,which were related to 5 traits such as plant height,1000-grain weight(1000-GW)and gra

关 键 词：小麦 关联分析 产量 钾效率 

分 类 号：S512.1[农业科学—作物学]