鸡传染性支气管炎病毒种子批的建立  

Establishment of seed batches of avian infectious bronchitis virus

作　　者：汪宏才[1] 汪辉[1] 王红琳[1] 温国元[1] 张腾飞[1] 罗玲[1] 卢琴[1] 张蓉蓉[1] 罗青平[1] WANG Hong-cai;WANG Hui;WANG Hong-Lin;WEN Guo-yuan;ZHNAG Teng-fei;LUO Ling;LU Qin;ZHANG Rong-rong;LUO Qing-ping(Institute of Animal Husbandry and Veterinary Sciences,Hubei Academy of Agricultural Sciences/Key Laboratory of Prevention and Control Agents for Animal Bacteriosis of Ministry of Agriculture and Rural Affairs/Hubei Key Laboratory of Animal Embryo and Molecular Breeding,Wuhan 430064,China)

机构地区：[1]湖北省农业科学院畜牧兽医研究所/农业农村部畜禽细菌病防治制剂创制重点实验室/动物胚胎工程及分子育种湖北省重点实验室,武汉430064

出　　处：《湖北农业科学》2020年第10期120-122,130,共4页Hubei Agricultural Sciences

基　　金：国家重点研发计划项目(2017YFD0500702);国家现代农业产业技术体系专项资金项目(CARS-41-G13);湖北省农业科技创新中心项目(2019-620-000-001-17)。

摘　　要：为保证传染性支气管炎抗原的质量,测定了3种不同来源的传染性支气管炎病毒株的EID50,选取滴度最高的病毒株作为毒种,建立了传染性支气管炎病毒WH株种子批,并对冻干毒种进行了毒价测定。结果表明,含毒尿囊液在冻干前后毒价无显著性差异,毒种的EID50在10代内无变化。冻干毒种在-40℃和-15℃保存1年,其毒价基本不变。经检验种子批未受到细菌及其他微生物的污染。In order to ensure the quality of infectious bronchitis antigen,the EID50 of infectious bronchitis virus strains from three sources were determined and the virus strains with high titer as virus species were selected,and the seed batch of infectious bronchitis virus WH strain were established.The poison value of freeze-dried seed poison was determined.The results showed that there was no significant difference in the poison value of toxic allantoic fluid before and after freeze-drying,and the EID50 of the virus had no change within 10 generations.The poison value of freeze-dried seed poison remained basically unchanged when it was stored at-40℃ and-15℃ for one year.The seed batch was not contaminated by bacteria and other microorganisms.

关 键 词：传染性支气管炎 WH株 种子批 

分 类 号：S852.5[农业科学—基础兽医学]