当归藤中总黄酮提取工艺优化及其含量测定  

Study on extraction technology optimization and content determination of total flavones in Embelia parviflora Wall

作　　者：卢森华 刘佳明 张凤懿 陈勇[3] 张志 LU Sen-hua;LIU Jia-ming;ZHANG Feng-yi;CHEN Yong;ZHANG Zhi(Yulin Center for Food and Drug Control,Yulin 537000,Guangxi,China;Luchuan County Center for Food and Drug Control of Yulin City,Yulin 537700,Guangxi,China;Guangxi University of Chinese Medicine,Nanning 530001,China;Liuzhou Hospital of Traditional Chinese Medicine,Liuzhou 545001,Guangxi,China)

机构地区：[1]玉林市食品药品检验检测中心,广西玉林537000 [2]玉林市陆川县食品药品检验检测中心,广西玉林537700 [3]广西中医药大学,南宁530001 [4]柳州市中医医院,广西柳州545001

出　　处：《湖北农业科学》2020年第10期123-126,共4页Hubei Agricultural Sciences

基　　金：国家自然科学基金项目(81660701);广西中医药大学自然科学研究项目面上项目(2015LX050)。

摘　　要：为优化当归藤(Embelia parviflora Wall)中总黄酮提取工艺,在单因素试验的基础上,以提取溶剂体积分数、料液比和提取时间为影响因素,采用L9(3^4)正交试验优化当归藤中总黄酮的最佳提取工艺。结果表明,提取溶剂体积分数、料液比和提取时间对当归藤中总黄酮提取率均有显著影响,优化后的当归藤中总黄酮的最佳提取工艺为50%乙醇溶液料,液比1∶200,提取时间120 min,芦丁质量浓度在9.16~54.96μg/mL(r=0.9998)范围内线性关系良好,平均回收率为97.6%(n=6),RSD为2.2%。To establish the extraction technology and content determination method of total flavonoids in Embelia parviflora Wall.On the basis of single factor experiment,the optimum extraction technology of total flavonoids from Embelia parviflora Wall was optimized by L9(3^4)orthogonal test,and the content of total flavonoids in Embelia parviflora Wall was determined.Results showed that the extraction solvent concentration,the ratio of material to liquid,and the extraction time all had significant effects on the extraction rate of total flavonoids from Embelia parviflora Wall.The optimum extraction technology of total flavonoids from Embelia parviflora Wall was material-liquid ratio of 1:200,and reflux extraction at 100℃ in water bath for 120 min.The linear relationship of rutin concentration was good in the range of 9.16~54.96μg/mL(r=0.9998).The average recovery was 97.6%(n=6),and RSD was 2.2%.

关 键 词：当归藤(Embelia parviflora Wall) 总黄酮 含量 提取工艺 

分 类 号：TQ461[化学工程—制药化工]