冠状病毒相关基因在IBD肠黏膜组织中的表达与COVID-19患病风险的相关性研究  

Correlation between the expression of coronavirus-related genes in the intestinal mucosa of IBD and the risk of COVID-19

作　　者：王宏刚[1] 杨晓钟[1] 吴尚农[1] 戴伟杰[1] 马天恒[1] 谢睿[1] 沈鹏[1] 李倩君[1] WANG Honggang;YANG Xiaozhong;WU Shangnong;DAI Weijie;MA Tianheng;XIE Rui;SHEN Peng;LIQianjun(Department of Gastroenterology,the Affiliated Huaian No.1 People’s Hospital of Nanjing Medical University,Huai’an 223300,China)

机构地区：[1]南京医科大学附属淮安第一医院消化科,江苏淮安223300

出　　处：《南京医科大学学报（自然科学版）》2020年第6期788-792,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省卫生健康委科研课题(H2018082);淮安市自然科学研究计划课题(HAB201926);淮安市第一人民医院转化医学创新团队科研项目(YZHT201905)。

摘　　要：目的:分析炎症性肠病(inflammatory bowel disease,IBD)患者肠黏膜的冠状病毒相关基因[血管紧张素转换酶2(angiotensin-converting enzyme 2,ACE2)、跨膜丝氨酸蛋白酶2(transmembrane protease serine 2,TMPRSS2)、二肽基肽酶4(dipeptidyl peptidase 4,DPP4)、氨基肽酶N(aminopeptidase N,ANPEP)]的表达差异。方法:收集新型冠状病毒肺炎(corona virus disease 2019,COVID-19)患者的腹泻发生率,并且利用IBDMDB数据库,分析IBD患者肠黏膜ACE2、TMPRSS2、DPP4、ANPEP的表达差异。结果:COVID-19患者的腹泻发生率为5.8%(136/2350)。与直肠黏膜相比,末端回肠ACE2、TMPRSS2、DPP4、ANPEP的差异表达倍数分别为9.800、0.571、5.318和11.127倍,差异有统计学意义(P<0.05)。但这4种基因的表达在IBD与非IBD患者中未显示出显著差异(P>0.05)。GO分类显示这些基因都参与了病毒进入宿主细胞的生物学过程,且各基因之间有相互作用。结论:冠状病毒相关基因(ACE2、TMPRSS2、DPP4、ANPEP)在IBD与非IBD的肠黏膜组织中的表达没有显著差异,推测IBD没有显著增加COVID-19的患病风险。Objective:The aim of this study was to analyze the expression of coronavirus-associated genes[angiotensin-converting enzyme 2(ACE2),transmembrane protease serine 2(TMPRSS2),dipeptidyl peptidase 4(DPP4),aminopeptidase N(ANPEP)]in the intestinal mucosa of patients with inflammatory bowel disease(IBD).Methods:The occurrence of diarrhea in patients with corona virus disease 2019(COVID-19)were collected,and the IBDMDB database was used to analyze the expression of ACE2,TMPRSS2,DPP4 and ANPEP in the intestinal mucosa of IBD patients.Results:The occurrence of diarrhea in COVID-19 patients was 5.8%(136/2350).Compared with the rectal mucosa,the difference expression fold of ACE2,TMPRSS2,DPP4 and ANPEP in the terminal ileum were 9.800,0.571,5.318 and 11.127,respectively,with statistical differences(P<0.05).However,the expression of these 4 genes did not show significant differences between IBD and non-IBD patients(P>0.05).The GO classification showed that these genes were involved in the biological process of the virus entering the host cell,and there was a network interaction between these genes.Conclusion:There is no significant difference in the expression of coronavirus-related genes(ACE2,TMPRSS2,DPP4,ANPEP)in the intestinal mucosa of IBD and non-IBD.We speculate that IBD patients do not have a significantly increased risk of COVID-19.

关 键 词：新型冠状病毒 COVID-19 炎症性肠病 基因 肠黏膜 

分 类 号：R512.99[医药卫生—内科学]