Max作用蛋白1-0在氧糖剥夺诱导SH-SY5Y细胞凋亡中的作用  

Roles of Mxi1-0 in apoptosis of SH-SY5Y cells induced by oxygen and glucose deprivation

作　　者：聂萍 胡圳圳[2] 袁雅 吴伟玲[1,3] 郑大同[1,2,3] NIE Ping;HU Zhenzhen;YUAN Ya;WU Weiling;ZHENG Datong(Department of Pediatrics,the Second Clinical Medical School,Nanjing Medical University,Nanjing 210011;Clinical Molecular Diognostic Center,the Second Affiliated Hospital of Nanjing Medical University,Nanjing 210003,China;Children’s Medical Center,the Second Affiliated Hospital of Nanjing Medical University,Nanjing 210003,China)

机构地区：[1]南京医科大学第二临床医学院儿科,江苏南京210011 [2]南京医科大学第二附属医院临床分子基因检测中心,江苏南京210003 [3]南京医科大学第二附属医院儿童医学中心,江苏南京210003

出　　处：《南京医科大学学报（自然科学版）》2020年第6期816-820,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省妇幼健康科研项目(F201711)。

摘　　要：目的:探讨Max作用蛋白1-0(max action protein 1-0,Mxi1-0)在氧糖剥夺(oxygen-glucose deprivation,OGD)诱导SH-SY5Y细胞凋亡中的作用。方法:利用Western blot检测SH-SY5Y细胞在OGD条件下Mxi1-0和线粒体自噬相关蛋白LC3-Ⅱ、p62、Tom20的表达水平。Mxi1-0 siRNA转染SH-SY5Y细胞,以CCK-8法和Annexin V/PI染色法检测Mxi1-0在OGD诱导细胞凋亡中的作用。结果:OGD条件下,SH-SY5Y细胞中Mxi1-0的表达先上升后下降。OGD处理使线粒体自噬相关蛋白LC3-Ⅱ表达升高,p62和Tom20蛋白表达下降。OGD条件下,Mxi1-0沉默可抑制OGD诱导的线粒体自噬,增强OGD诱导的SH-SY5Y细胞凋亡。结论:Mxi1-0通过增强线粒体自噬拮抗OGD诱导的SH-SY5Y细胞凋亡。Objective:This study aims to investigate the role of max action protein 1-0(Mxi1-0)in SH-SY5 Y cell apoptosis induced by oxygen-glucose deprivation(OGD).Methods:The expression levels of Mxi1-0 and mitophagy related proteins LC3-Ⅱ,p62,Tom20 in SH-SY5 Y cells under OGD conditions were detected by Western blot.Mxi1-0 siRNA was tranfected into SH-SY5 Y cells.The expression of Mxi1-0 and mitophagy related proteins(LC3-Ⅱ,p62,Tom20)were detected in SH-SY5 Y cells under OGD using Western blot.The role of Mxi1-0 in OGD-induced apoptosis was detected using CCK-8 method and Annexin V/PI staining.Results:The expression of Mxi1-0 increased firstly and then decreased in SH-SY5 Y cells after OGD treatment.The expression of mitophagy related protein LC3-Ⅱincreasesed and the expression of p62 and Tom20 proteins decreased after OGD treatment.Under OGD conditions,Mxi1-0 silence reduced mitophagy in SH-SY5 Y induced by OGD,and increase the apoptosis of SH-SY5 Y induced by OGD.Conclusion:Mxi1-0 antagonizes OGD induced apoptosis of SH-SY5 Y cells by enhancing mitochondrial autophagy.

关 键 词：Max作用蛋白1-0 氧糖剥夺 细胞凋亡 线粒体自噬 SH-SY5Y细胞 

分 类 号：R320.54[医药卫生—人体解剖和组织胚胎学] R743.3[医药卫生—基础医学]