苏云金杆菌Bt-59菌株Cry/Cyt毒素蛋白鉴定及杀虫活性  

Characteristics of Cry and Cyt Proteins and Insecticidal Activity in Bacillus thuringiensis Bt-59

作　　者：韩光杰[1,2] 徐灵环 李传明 刘琴[1] 彭琦[2] 徐彬[1] 宋福平[2] 徐健[1] HAN Guangjie;XU Linghuan;LI Chuanming;LIU Qin;XU Bin;SONG Fuping;XU Jian(Jiangsu Lixiahe District Institute of Agriculture Sciences/National Agricultural Experimental Station for Agricultural Microbiology,Yangzhou 225007,China;State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China)

机构地区：[1]江苏里下河地区农业科学研究所/国家农业微生物扬州观测实验站,扬州225007 [2]中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室,北京100193

出　　处：《中国生物防治学报》2020年第3期458-464,共7页Chinese Journal of Biological Control

基　　金：国家自然科学基金(31071740);植物病虫害生物学国家重点实验室开放课题(SKLOF201807);省部共建国家重点实验室培育基地江苏省食品质量安全重点实验室开放课题(028074911709)。

摘　　要：苏云金杆菌以色列亚种(Bacillus thuringiensis subsp. israelensis,Bti)是防治蚊幼虫的重要病原细菌,不同菌株间毒素蛋白种类和分布不尽相同。本研究利用苏云金杆菌以色列亚种Bt-59菌株的全基因组数据,对潜在的毒素基因种类、分布及菌株杀虫活性进行了分析。结果表明,Bt-59菌株除了含有Bti标准菌株主要毒素基因外,还含有Cry4-like基因,且Cry10Aa和Cyt1Ca基因在质粒4上发生了易位。质粒5上的Cry60Aa和Cry60Ba基因编码的毒素蛋白含有ETXMTX2结构域,并与苏云金杆菌jegathesan亚种的同源蛋白一致性为100%。Cyt1Ca蛋白除了含有Bacthurtoxin结构域外,还含有2个串联的RicinBlectin2结构域。菌株Bt-59对蚊幼虫具有较高的杀虫活性,对白纹伊蚊Aedes albopictus和淡色库蚊Culex pipiens pallens的LC50分别为1.239μL/L和0.341μL/L。研究结果明确了菌株Bt-59毒素蛋白的种类和特征,为菌株遗传改良提供理论基础。Bacillus thuringiensis subsp. israelensis(Bti) is an important pathogen for control of mosquito larvae. The types and distribution of toxin proteins are diverse among different strains. In this study, the types and characteristics of toxin genes and insecticidal activity of Bt-59 strain were determined. Bt-59 is different from Bti standard strains in that it harbors a Cry4-like gene and the positions of Cry10Aa and Cyt1Ca are translocated on plasmid 4. The toxin proteins encoded by Cry60Aa and Cry60Ba genes on plasmid 5 contain the ETXMTX2 domain, showing identity of 100% to homologous proteins of B. thuringiensis subsp. jegathesan. Besides the Bacthurtoxin domain, Cyt1Ca protein also contains two RicinBlectin2 domains in tandem. Bt-59 has high insecticidal activity against mosquito larvae, with LC50 for Aedes albopictus and Culex pipiens pallens of 1.239 μL/L and 0.341 μL/L, respectively. Our research provides a theoretical basis for the genetic improvement of the Bt-59 strain.

关 键 词：苏云金杆菌 Cry蛋白 Cyt蛋白 蚊子 生物防治 

分 类 号：Q933[生物学—微生物学]