TGF-β诱导的linc01503促进食管鳞状细胞癌细胞的增殖、侵袭及EMT过程  

TGF-βinduced lncRNA linc01503 promotes proliferation,invasion and EMT process of esophageal squamous cell carcinoma cells

作　　者：郭艳丽[1] 牛云峰 梁晓亮[1] 郭炜[1] 沈素朋[1] 梁佳[1] 董稚明[1] GUO Yanli;NIU Yunfeng;LIANG Xiaoliang;GUO Wei;SHEN Supeng;LIANG Jia;DONG Zhiming(Hebei Cancer Institute,the Fourth Hospital of Hebei Medical University,Shijiazhuang 050011,Hebei,China)

机构地区：[1]河北医科大学第四医院河北省肿瘤研究所,河北石家庄050011

出　　处：《中国肿瘤生物治疗杂志》2020年第7期764-769,共6页Chinese Journal of Cancer Biotherapy

基　　金：河北省自然科学基金资助项目(No.H2019206664);河北省医学科学研究重点课题计划项目(No.20170698);河北省人才工程培养资助项目(No.201901035)。

摘　　要：目的:探讨长链非编码RNA(long noncoding RNA,lncRNA)linc01503在食管鳞状细胞癌(esophageal squamous cell cancer,ESCC)组织和细胞中的表达及其对ESCC细胞增殖、侵袭及上皮间质转化(epithelial-mesenchymal transition,EMT)的影响。方法:选取2012年1月至2014年12月河北医科大学第四医院收治的119例ESCC患者的癌及癌旁组织标本,应用qPCR法检测ESCC组织及ESCC细胞系(Kyse150、Kyse170、Eca109、TE1和TE13)中linc01503的表达水平;分别用pGPU6-shRNAlinc01503转染、TGF-β1处理ESCC细胞,用qPCR法检测转染前后EMT相关基因及处理前后linc01503表达的变化,用MTS及Transwell小室法检测linc01503对ESCC细胞增殖及侵袭的影响。结果:linc01503在ESCC组织和细胞系中高表达(均P<0.05),ESCC组织中linc01503高表达与患者淋巴结转移、浸润深度、TNM分期有关联,并与生存期密切相关(均P<0.05)。TGF-β处理促进ESCC细胞的EMT过程,同时诱导linc01503表达明显上调。linc01503敲低明显抑制细胞的增殖及侵袭能力(均P<0.05),同时明显增加了E-cadherin的表达而降低了N-cadherin、vimentin基因的表达(均P<0.05)。结论:linc01503作为TGF-β的下游效应基因之一,促进了ESCC细胞的增殖、侵袭及EMT过程。Objective:To investigate the expression of lncRNA linc01503 in esophageal squamous cell carcinoma(ESCC)tissues and cell lines,as well as its effect on the proliferation,invasion and epithelial-mesenchymal transition(EMT)of ESCC cells.Methods:A total of 119 pairs of tumor tissues and corresponding para-cancerous tissues of ESCC patients were obtained from the Fourth Affiliated Hospital of Hebei Medical University between Jan.2012 and Dec.2014.The expression of linc01503 in ESCC tissues and cell lines(Kyse150,Kyse170,Eca109,TE1 and TE13)was detected by qPCR.The ESCC cells were transfected with pGPU6-shRNA-linc01503 or treated with TGF-β.The expressions of EMT related genes before and after transfection as well as linc01503 expression before and after TGF-βtreatment were detected with qPCR.MTS and Transwell assay were performed to assess the effect of linc01503 on prolifer‐ation and invasion of ESCC cells.Results:The expression of linc01503 was significantly elevated in ESCC tissues and cell lines(all P<0.05).High expression of linc01503 was correlated with lymph node metastasis,depth of infiltration,TNM stage and the survival of ESCC patients(all P<0.05).Treatment with TGF-βpromoted EMT of ESCC cells and induced a significant up-regulation of linc01503 expression.Knockdown of linc01503 significantly inhibited proliferation and invasion ability of ESCC cells;Meanwhile,the low expression of linc01503 increased the expression of E-cadherin but decreased the expressions of N-cadherin and vimentin(all P<0.05).Conclusion:lncRNA linc01503,as one of the downstream effect genes of TGF-β,promotes the proliferation,invasion and EMT process of ESCC cells.

关 键 词：食管鳞状细胞癌 长链非编码RNA linc01503 转化生长因子-Β 增殖 侵袭 上皮间质转化 

分 类 号：R735.1[医药卫生—肿瘤] R730.2[医药卫生—临床医学]