欧洲云杉PSⅡ的热稳定性对温度升高的响应  

Response to Thermal Stability of PSⅡfor Temperature Rising in Picea abies

作　　者：贾子瑞[1,2] 王亚 马建伟 安三平 胡继文 王军辉 Jia Zirui;Wang Ya;Ma Jianwei;An Sanping;Hu Jiwen;Wang Junhui(Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091;State Key Laboratory of Forest Genetics and Breeding,Beijing 100091;Research Institute of Forestry of Xiaolongshan,Gansu Province,Tianshui 741020;Key Laboratory of Secondary Forest Cultivation in Gansu Province,Tianshui 741020)

机构地区：[1]中国林业科学研究院林业研究所,北京100091 [2]林木遗传育种国家重点实验室,北京100091 [3]甘肃省小陇山林业科学研究所,天水741020 [4]甘肃省次生林培育重点实验室,天水741020

出　　处：《林业科学》2020年第7期22-32,共11页Scientia Silvae Sinicae

基　　金：中央级公益性科研院所基本科研业务费专项资金项目“欧洲云杉高温胁迫下PSⅡ基因调控机理”(CAFYBB2018ZY001-7);中央级公益性科研院所基本科研业务费专项资金项目“应用2b-rad技术研究红皮云杉与近缘种的遗传分化”(CAFYBB2016QB006)。

摘　　要：【目的】以欧洲云杉5年生幼苗为对象,研究其PSⅡ热激后自我修复能力,评估它的PSⅡ在未来气候变暖情况下的热稳定性和热损伤的自我修复能力。【方法】选择3个无性系、每个无性系10个分生株,共计30个分生株,放入人工气候室正常条件下培养,2周后应用hand-PEA和image-PAM检测叶片的荧光参数和影像作为对照。放入38℃培养箱中在低光的条件下预热5 h后,在40、42、43、44、46、48、50、52、53和54℃下分别热激30 mins,每个处理3个无性系(每个无性系1个分生株),应用hand-PEA和image-PAM检测叶片PSⅡ的荧光参数和影像,筛选2个胁迫临界温度。将热激后选择的2个临界温度处理的幼苗,再次放入人工气候室正常条件下培养2周,用hand-PEA和image-PAM继续检测叶片PSⅡ的荧光参数和影像。【结果】1)通过对参数Y(Ⅱ)、Y(NO)和Y(NPQ)荧光影像和动力学曲线研究发现,欧洲云杉在44℃热激胁迫下,达到初始胁迫温度,52℃热激胁迫下达到最大胁迫温度,与草本、木本以及近缘物种植物相比,欧洲云杉PSⅡ的热稳定性都处于较高水平。2)应用image-PAM检测临界温度下PSⅡ荧光参数F0、Fm、Fv/Fm、NPQ、qL和qP的影像和NPQ、qL和qP荧光动力学参数曲线:44℃短时热激下Fm和Fv/Fm影像减弱,qL和qP荧光影像增强,NPQ、qL和qP荧光动力学参数曲线高于对照;52℃短时热激下Fm和NPQ被增强,Fv/Fm、qL和qP影像被减弱,NPQ、qL和qP的荧光动力学参数曲线低于对照。3)应用hand-PEA进一步检测PSⅡ荧光参数量值的变化,44℃短时热激下,Fv/Fm、OJIP曲线都没有明显变化,仅PIABS降至对照组的79.5%,叶绿素(Chl)吸收和捕获光能的能力、电子的传递、以及电子传递链中心蛋白的活性都没受到明显的抑制,并促进天线色素(Chl)吸收能量(ABC)的光化学反应的能力和荧光淬灭的能力;而52℃短时热激下,PSⅡ的功能已发生明显的抑制,OJIP曲线也出现大幅度的“K[Objective]In this study,5-year-old European spruce(Picea abies)seedlings were used to evaluate the thermal stability and self-repairing ability of PSⅡin the future climate warming.[Method]Three clones,10 ramets of each clone,30 ramets in total,were selected and cultured in an artificial climate chamber under normal conditions.Two weeks later,hand-PEA and image-PAM were used to detect the fluorescence parameters and image of leaves as control.After preheating for 5 hours in a low light incubator at 38℃,seedlings were heated for 30 minutes at 40,42,43,44,46,48,50,52,53 and 54℃,respectively.Three clones(one ramet per clone)were treated in each experimental group.Hand-PEA and image-PAM were used to detect the fluorescence parameters of leaves and two critical temperatures of tress were selected.Seedlings treated under two critical temperatures were cultured in an artificial climate chamber for two weeks.Hand-PEA and image-PAM were used to detect the fluorescence parameters and images of leaves,and to study the self-repairing ability of spruce PSⅡafter heat shock.[Result]1)Through the study of Y(Ⅱ),Y(NO)and Y(NPQ)fluorescence images and kinetic curves,it was found that under 44℃heat stress,PSⅡof P.abies reached the initial stress temperature,and under 52℃heat stress,it reached the maximum stress temperature.Compared with herbs,other woody species and related species,the thermal stability of PSⅡwas at a higher level for P.abies.2)The fluorescence parameters of F0,Fm,Fv/FM,NPQ,qL and qP of PSⅡand the fluorescence kinetic parameters of NPQ,qL and qP were detected by image-PAM at critical temperature:the fluorescence kinetic parameters of Fm and Fv/Fm were weakened,the fluorescence images of qL and qP were enhanced,and the fluorescence kinetic parameters of NPQ,qL and qP were higher than those of the control at 44℃.The fluorescence kinetic curves of Fv/Fm,qL and qP were lower than those of the control.3)The hand-PEA was used to further detect the change of fluorescence parameters of PSⅡ.Under 44�

关 键 词：欧洲云杉 PSⅡ 热激胁迫 荧光参数和影像 临界温度 

分 类 号：S718.43[农业科学—林学]