甲型流感病毒感染者CD160^+CD8^+T淋巴细胞亚群分析  

Analysis of CD160+CD8+ lymphocyte subsets of influenza A virus infectors

作　　者：徐晓雪 王蓓蓓 宋蕊 宋洋子 赵雪[1] 姜钰 曹钰 曾辉[1] Xu Xiaoxue;Wang Beibei;Song Rui;Song Yangzi;Zhao Xue;Jiang Yu;Cao Yu;Zeng Hui(Peking University Ditan Teaching Hospital,100015 Beijing,China;Beijing Ditan Hospital,Capital Medical University,100015 Beijing,China)

机构地区：[1]北京大学地坛医院教学医院传染病学研究所,北京100015 [2]首都医科大学附属北京地坛医院传染病学研究所,北京100015

出　　处：《中华实验和临床感染病杂志（电子版）》2020年第3期206-211,共6页Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition)

基　　金：国家重点研发计划(No.2016YFC1200805);北京市留学人员科技项目择优资助(优秀类)项目;2017年新发突发传染病研究北京市重点实验室开放研究课题(No.DTKF201701);2017年北京地坛医院院内基金(No.DTZLX201709)。

摘　　要：目的研究甲型流感病毒(IAV)感染者外周血中CD160^+CD8^+T淋巴细胞亚群的特征。方法收集2018年12月至2019年3月流感季就诊于北京大学地坛医院教学医院感染二科IAV感染者共37例及同期体检的健康对照者51例,应用多色流式细胞检测平台,检测外周血不同分化阶段CD160^+CD8^+T淋巴细胞的比例。结果与健康对照相比,IAV感染者外周血T细胞中CD160^+CD8^+T细胞比例下降[24.50%(14.70%,44.10%)vs.8.85%(5.14%,18.15%)],差异有统计学意义(U=326.00、P<0.001)。IAV感染者与健康对照相比,其CD8^+T中初始T细胞(TN)[3.88%(1.28%,9.48%)vs.0.22%(0.12%,0.60%)]、中央记忆性T细胞(TCM)[8.70%(4.43%,15.80%)vs.1.51%(0.69%,2.54%)]、效应记忆性T细胞(TEM)[22.30%(13.80%,30.40%)vs.7.71%(5.23%,16.25%)]和终末效应记忆T细胞(TEMRA)[(46.99±22.91)%vs.(21.60±13.38)%]4个亚群中CD160^+细胞的比例均下降,差异有统计学意义(U=238.50、81.50、412.00,t=6.03;P均<0.001)。IAV感染者出院时较入院时CD160^+CD8^+T细胞比例上升[(8.92±7.84)%vs.(16.40±9.43)%](t=4.09、P=0.001),但仍低于正常水平[24.50%(14.70%,44.10%)vs.(16.40±9.43)%],差异有统计学意义(U=209.50、P=0.0029)。结论甲型流感病毒感染者外周血中T淋巴细胞CD160^+CD8^+T细胞亚群比例显著降低,可能是免疫失衡的重要表现。Objective To investigate the characteristics of CD160^+CD8^+ T lymphocyte subsets inperipheral blood of patients with influenza A virus (IAV) infection. Methods Total of 37 patients with IAVinfection and 51 healthy controls in the same period were recruitmented in Peking University Ditan TeachingHospital from December 2018 to March 2019, and the frequencies of CD160^+CD8^+ T cells in peripheral bloodof different differentiation stages were detected by multicolor flow cytometry. Result Compared with healthcontrols, the frequencies of CD160^+CD8^+ T cells in peripheral blood of patients with IAV infection decreased[24.50% (14.70%, 44.10%) vs. 8.85% (5.14%, 18.15%)], with significant difference (U = 326.00, P < 0.001).Compared with the controls, these decrease occurred in the four subsets of CD8^+ T cells that include thenaive T cells (TN) [3.88% (1.28%, 9.48%) vs. 0.22% (0.12%, 0.60%)], central memory T cells (TCM) [8.70%(4.43%, 15.80%) vs. 1.51% (0.69%, 2.54%)], effector memory T cells (TEM) [22.30% (13.80%, 30.40%) vs.7.71% (5.23%, 16.25%)] and effective memory re-expressing T cells (TEMRA) [(46.99 ± 22.91) % vs. (21.60 ±13.38) %] of cases with IAV infection, with significant differences (U = 238.50, 81.50, 412.00, t = 6.03;allP < 0.001). The proportion of CD160^+CD8^+ T cells in the discharged patients with IAV infection was higherthan that of admission [(8.92 ± 7.84) % vs. (16.40 ± 9.43) %], with significant difference (t = 4.09, P = 0.001),but still lower than that of health controls [24.50% (14.70%,44.10%) vs (16.40 ± 9.43) %], with significantdifference (U = 209.50, P = 0.0029). Conclusions The frequency of CD160^+CD8^+ T cell subsets in peripheralblood T lymphocytes of patients with IAV infection decreased significantly, and may be an importantmanifestation of immune imbalance.

关 键 词：CD160共抑制分子 多色流式 季节性甲型流感 T淋巴细胞 

分 类 号：R511.7[医药卫生—内科学]