金线莲灰霉病病害鉴定  

Identification of the Pathogen of Grey Mold on Anoectochilus roxburghii

作　　者：刘惠英 杨世福 赵志博 魏蜜[1,2] 熊玉洁 易璐瑶 LIU Huiying;YANG Shifu;ZHAO Zhibo;WEI Mi;XIONG Yujie;YI Luyao(College of Life Science and Technology,Hubei Key Laboratory of Quality Control of Characteristic Fruits and Vegetables,Hubei Engineering University,Xiaogan 432000,China;School of Life Science,Hubei University,Wuhan 430062,China;College of Agriculture,Guizhou University,Guiyang 550025,China;College of Life Science&Technlogy,Huazhong University of Science and Technoloy,Wuhan 430070,China)

机构地区：[1]湖北工程学院生命科学技术学院,特色果蔬质量安全控制湖北省重点实验室,湖北孝感432000 [2]湖北大学生命科学学院,湖北武汉430062 [3]贵州大学农学院,贵州贵阳550025 [4]华中科技大学生命科学与技术学院,湖北武汉430074

出　　处：《云南农业大学学报（自然科学版）》2020年第4期601-607,共7页Journal of Yunnan Agricultural University:Natural Science

基　　金：国家自然科学基金项目(31600055);中国博士后项目(2018M642813)。

摘　　要：【目的】明确云南省金线莲种植基地金线莲灰霉病病原菌。【方法】采用组织分离法对病原菌进行分离纯化,并完成柯赫氏法则验证。根据病原菌的形态特征、rDNA-ITS和RPB2基因序列明确病菌种类。【结果】从发病组织分离获得纯培养病原真菌C2L2能导致金线莲发生灰霉病;根据形态特征与分子系统进化分析,将该病原菌确定为灰葡萄孢(Botrytis cinerea)。【结论】本研究为金线莲病害研究与防控提供参考依据。[Purpose]To clarify the pathogen of gray mold on Anoectochilus roxburghii in the planting base of A. roxburghii in Yunnan Province.[Method]The pathogens were isolated and purified by tissue separation, and the pathogenicity test of the pathogen was verified by Koch’s postulates. The pathogen species were identified based on the morphological characteristics, rDNA-ITS and RPB2 gene sequences of the pathogen.[Result]Pure cultured C2L2 was isolated from diseased tissues, which can cause symptoms of gray mold on A. roxburghii. According to morphological characteristics and molecular phylogenetic analysis, the pathogen was identified as Botrytis cinerea.[Conclusion]This study result provided a reference for disease research and prevention.

关 键 词：金线莲 灰霉病 灰葡萄孢 鉴定 

分 类 号：S435.621[农业科学—农业昆虫与害虫防治]