水稻显性感光抑制基因Su-E1(t)的定位及遗传分析  

Genetic Mapping of a Dominant Photosensitive Suppresser Gene,Su-E1(t)in Oryza Sativa

作　　者：周振玲[1] 孙志广 徐卫平 徐艳[1] 杨波[1] 刘艳[1] 王宝祥[1] 江玲[2] 徐大勇[1,2] ZHOU Zhen-ling;SUN Zhi-guang;XU Wei-ping;XU Yan;YANG Bo;LIU Yan;WANG Bao-xiang;JIANG Ling;XU Da-yong(Lianyungang Academy of Agricultural Sciences,Lianyungang 222000;Agricultural College of Nanjing Agricultural University,Nanjing 210095)

机构地区：[1]连云港市农业科学院,连云港222000 [2]南京农业大学农学院,南京210095

出　　处：《植物遗传资源学报》2020年第4期945-953,共9页Journal of Plant Genetic Resources

基　　金：江苏省政策引导类计划(苏北科技专项)(LYG-SZ201930);江苏省六大人才高峰项目(NY214);连云港市财政专项(QNJJ1803);现代农业技术体系建设专项资金资助项目(CARS-01-61)。

摘　　要：利用粳稻品种Asominori和籼稻品种IR24衍生的重组自交系群体,在南京和海南2种自然环境下对水稻抽穗期QTL进行检测,分别检测到5个和6个影响抽穗期的QTL,其中位于第6染色体的qDTH-6在2种环境下都能被检测到,LOD值分别为6.28和12.93,贡献率分别为12.26%和17.18%。对以Asominori为背景、在qDTH-6处置换了IR24片段的染色体片段置换系CSSL45及背景亲本进行人工短日照处理,发现qDTH-6来自籼稻IR24的等位基因具有显性感光抑制效应。利用抽穗期基因型测验系和9311等一些常规稻与CSSL45杂交分析,进一步证实了qDTH-6的显性感光抑制功能,并初步判断其抑制对象为主效感光基因E1(Ghd7),本研究将其命名为Su-E1(t)。同时利用CSSL45×Asominori次级F2群体分别在人工短日照和自然长日照条件下对Su-E1(t)进行了进一步定位,将其定位在SSR标记RM527附近。本研究对有效地解决水稻籼粳亚种间杂种生育期超亲晚熟的难题具有重要意义。The recombinant inbred line population,which was derived from a hybridization of the japonica variety Asominori and the indica variety IR24,was deployed for the quantitative trait locus(QTL)mapping for the genetic components underlying the heading date(HD)in two environments(Nanjing and Hainan,both from China).As a result,five and six QTLs were detected from both locations respectively.Of them,this locus qDTH-6 on chromosome 6 could be simultaneously detected with LOD values of 6.28 and 12.93,and with the contribution rates of 12.26%and 17.18%,respectively.Moreover,this line CSSL45,which is one of IR24 chromosome segment substitution lines(CSSL)and which contains qDTH-6,and its background parental line were treated under short-day(SD)condition.The qDTH-6 allele from IR24 showed dominant photo-inhibition.By analyzing a set of heading date tester lines and some tester cultivars crossed with CSSL45,we speculated that the qDTH-6 allele from IR24,which names Su-E1(t),inhibited the function of photoperiod-sensitive gene E1(Ghd7).Using CSSL45×Asominori secondary F2 population,Su-E1(t)was further localized close to SSR marker RM527.Taken together,the results might be of significance in order to shorten the heading date of indica-japonica hybrid.

关 键 词：水稻 抽穗期 QTL 感光抑制基因 

分 类 号：S511[农业科学—作物学]