小麦-中间偃麦草6J^S/6B代换系的分子细胞学鉴定  

Molecular and Cytogenetic Identification of WheatT.intermedium 6J^S/6B Substitution Line

作　　者：罗小军 乔麟轶 李欣[2] 李光蓉[3] 郭慧娟 阎晓涛 张树伟 常利芳 闫金龙[4] 畅志坚 张晓军 LUO Xiao-jun;QIAO Lin-yi;LI Xin;LI Guang-rong;GUO Hui-juan;YAN Xiao-tao;ZHANG Shu-wei;CHANG Li-fang;YAN Jin-long;CHANG Zhi-jian;ZHANG Xiao-jun(College of Bio-engineering,Shanxi University,Taiyuan 030006;College of Agronamy,Shanxi Agricultural University/Shanxi Key Laboratory of Crop Genetics and Molecular Improvement/Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau of Ministry of Agriculture,Taiyuan 030031;College of Life Science and Technology,University of Electronic Science and Technology of China,Chengdu 610054;Millet Research Institute,Shanxi Academy of Agricultural Sciences,Changzhi 046011)

机构地区：[1]山西大学生物工程学院,太原030006 [2]山西农业大学农学院/作物遗传与分子改良山西省重点实验室/农业部黄土高原作物基因资源与种质创制重点实验室,太原030031 [3]电子科技大学生命科学与技术学院,成都610054 [4]山西省农业科学院谷子研究所,长治046011

出　　处：《植物遗传资源学报》2020年第4期984-990,共7页Journal of Plant Genetic Resources

基　　金：国家重点研发计划(2016YFD0102004);山西省重点研发计划(201803D221018-5,201703D211007,201803D421020);山西省农业科学院项目(YGG17123,YCX2018D2YS01);山西省重点科技创新平台(201605D151002)。

摘　　要：将近缘植物的抗病基因导入小麦是改良小麦抗病性的重要途径之一,对其外源染色体进行准确鉴定能够提高外源基因的选择与利用效率。本研究分别利用小麦白粉病、条锈病菌生理小种接种、荧光原位杂交和分子标记的方法对来源于中间偃麦草的部分双二倍体TAI7047为中间亲本创制的新种质CH357进行了鉴定分析。结果显示,CH357是一个小麦-中间偃麦草6JS/6B代换系,兼抗小麦白粉病、条锈病2种病害,其抗性可能来源于中间偃麦草的6JS染色体,可以作为一个小麦白粉病和条锈病新抗源进行小麦抗性遗传改良。基于中间偃麦草第6同源群Contig序列开发了160个STS标记,其中8个可作为识别小麦-中间偃麦草异代换系CH357中6JS染色体/片段的特异标记,为中间偃麦草6JS染色体/片段的鉴定提供较为经济和方便的检测手段。Transforming resistance genes of wild relatives into elite cultivars is an effective approach to improve the wheat disease resistance.Identification of the alien chromosomes by deployment of a rapid and precise method becomes important in order to simplify the process on selection and utilization of the favorable genes.In this study,CH357,a new wheat germplasm resource which was derived from wheat-Thinopyrum intermedium(Host)Barkworth&D.R.Dewey partial amphiploid TAI7047,was subjected for tests upon infections of Powdery mildew and Stripe rust,as well as the evaluation by fluorescent in situ hybridization(FISH)and molecular markers detection.The results showed that CH357 contained a wheat-T.intermedium(Host)Barkworth&D.R.Dewey 6 JS/6 B chromosome substitution.This genotype was tested to be resistant against wheat powdery mildew and stripe rust,and the resistances were likely caused by 6 JS chromosome of T.Intermedium(Host)Barkworth&D.R.Dewey,thus raising a potential for wheat resistance breeding.Furthermore,160 STS markers were developed based on the Contigs sequences of T.intermedium(Host)Barkworth&D.R.Dewey group 6 genome.Out of that,eight markers were found to specifically amplify the alien chromosome of CH357.The PCRbased assay will provide a user-friendly method for identifying the 6 JS chromosome or fragments of T.intermedium(Host)Barkworth&D.R.Dewey in wheat.

关 键 词：中间偃麦草 代换系 染色体 STS 分子标记 

分 类 号：S512.1[农业科学—作物学]