紫薇属及近缘属13种植物基因组大小测定  

Genome Size Determination of 13 Taxa of Lagerstroemia L. and Two Closely Related Genera

作　　者：刘婷婷[1] 梁晓涵 张烨 程堂仁[1] 王佳[1] Mengmeng Gu 张启翔[1] 潘会堂[1] LIU Ting-ting;LIANG Xiao-han;ZHANG Ye;CHENG Tang-ren;WANG Jia;Mengmeng Gu;ZHANG Qi-xiang;PAN Hui-tang(Beijing Key Laboratory of Ornamental Plants Germplasm Innovation&Molecular Breeding/National Engineering Research Center for Floriculture/Beijing Laboratory of Urban and Rural Ecological Environment/College of Landscape Architecture,Beijing Forestry University,Beijing 100083;Department of Horticultural Sciences,Texas A&M AgriLife Extension,College Station,TX 77843-0100,USA)

机构地区：[1]花卉种质创新与分子育种北京市重点实验室/国家花卉工程技术研究中心/城乡生态环境北京实验室/北京林业大学园林学院,北京100083 [2]Department of Horticultural Sciences,Texas A&M AgriLife Extension,College Station,TX 77843-0100,USA

出　　处：《植物遗传资源学报》2020年第4期1020-1029,共10页Journal of Plant Genetic Resources

基　　金：“十三五”国家重点研发计划课题(2019YFD1001004);北京市科技计划课题(Z181100002418006)。

摘　　要：为探究千屈菜科紫薇属(Lagerstroemia L.)、黄薇属(Heimia Link)及千屈菜属(Lythrum L.)之间的亲缘关系,本研究利用流式细胞术以二倍体白菜(Brassica rapa Rupr.)和黄瓜(Cucumis sativus L.)为内标对紫薇属8种2品种、黄薇属2种和千屈菜属1种进行了基因组大小测定,利用染色体压片技术获得2种黄薇属植物和千屈菜的染色体核型参数。结果表明,10份紫薇属植物的基因组大小在341.00±2.00~370.00±8.89 Mbp之间;黄薇(Heimia myrtifolia Cham. et Schlechtend.)和柳叶黄薇(H. salicifolia Link)基因组分别为414.67±5.77 Mbp和420.00±7.00 Mbp,染色体数目均为16,核型公式分别为2n=16=6sm+8m+2st和2n=16=2sm+14m;千屈菜(Lythrum salicaria L.)的基因组为1294.00±30.32 Mbp,染色体数目为54,核型公式为2n=54=31sm+15m+8st。本研究首次报道了千屈菜科13种植物的基因组大小,以及2种黄薇属植物和千屈菜的核型,研究结果为千屈菜科植物基因组学和遗传进化研究奠定了基础。In order to explore the genetic relationship among Lagerstroemia L.,Heimia L. and Lythrum L. of the family Lythraceae,the genome sizes of eight species and two cultivars of Lagstroemia L.,of two species of Heimia L. and of Lythrum salicaria L. were estimated by flow cytometry method with Brassica rapa Rupr. and Cucumis sativus L. as inner calibration. The chromosome number and karyotype parameters of the two Heimia L. species and of Lythrum salicaria L. were obtained using traditional chromosome tableting. The results showed that the average genome size of Lagerstroemia L. species and cultivars ranged from 341.00±2.00 to 370.00±8.89 Mbp. The average genome size of Heimia myrtifolia Cham. et Schlechtend. and H. salicifolia Link were 414.67±5.77 Mbp and 420.00±7.00 Mbp respectively,and the karyotype formulas of the two species were 2 n=16=6 sm+8 m+2 st and 2 n=16=2 sm+14 m. The average genome size of Lythrum salicaria L. was 1294.00±30.32 Mbp,and the karyotype formula was 2 n=54=31 sm+15 m+8 st. In this study,the genome size of 13 taxa in the Lythraceae,and the karyotypes of two Heimia L. species and of L. salicaria L. were reported for the first time. The results of our research laid a foundation for research on genomics and genetics of the Lythraceae.

关 键 词：紫薇属 黄薇属 千屈菜属 基因组大小 染色体 流式细胞术 

分 类 号：S685.99[农业科学—观赏园艺]