红叶乌桕组培育苗关键技术研究  

Study on the Key Techniques for Tissue Culture Propagation of Euphorbia cotinifolia

作　　者：舒婷[1] SHU Ting(Forestry Science and Technology Test Center of Fujian Province,Zhangzhou 363600,Fujian,China)

机构地区：[1]福建省林业科技试验中心,福建漳州363600

出　　处：《安徽林业科技》2020年第3期9-12,共4页Anhui Forestry Science and Technology

基　　金：福建省林业科研项目(闽林科便函〔2018〕26号)。

摘　　要：选用红叶乌桕嫩枝为外植体,通过调整消毒时间和优化培养基,研究红叶乌桕组培育苗技术。结果表明,外植体先用75%的酒精消毒20 s,再用0.1%的HgCl2消毒8 min,效果最佳;红叶乌桕最佳的启动培养基是改良MS+6-BA 0.5 mg/L+NAA 0.2 mg/L;继代培养基以改良MS+6-BA 1.0 mg/L+NAA 0.2 mg/L效果最好,增殖系数达4.01,同时在增殖过程中加入核黄素0.5 mg/L和L-半胱氨酸0.5 mg/L,可以有效抑制褐化现象;生根培养基以1/2改良MS+NAA 1.0 mg/L+IBA 0.1 mg/L效果最佳,其生根率为96.6%;将生根苗移栽到泥炭土∶蛭石∶珍珠岩=3∶1∶1(体积比)的育苗基质中,成活率达92.5%。In this paper, twigs of Euphorbia cotinifolia were used as explants to study the key techniques for tissue culture propagation of Euphorbia cotinifolia by adjusting the disinfection time and optimizing the culture medium. The results showed that the best effect was achieved when the explants were disinfected with 75% ethyl alcohol for 20 s, followed by 0.1% HgCl2 for 8 min;the optimum initiation medium for Euphorbia cotinifolia was the improved MS +6-BA 0.5 mg/L +NAA 0.2 mg/L;the optimum subculture medium was the improved MS+6-BA 1.0 mg/L+NAA 0.2 mg/L with the multiplication coefficient reaching 4.01 and the browning in the multiplication process could be effectively inhibited with addition of 0.5 mg/L riboflavin and 0.5 mg/L L-cysteine;the optimum rooting medium was 1/2 the improved MS+NAA1.0 mg/L+IBA 0.1 mg/L with a rooting rate of 96.6%;the survival rate of the rooted plantlets transplanted in the plantlet-raising medium with volume ratio of peat soil, vermiculite and pearlite being3:1:1 reached 92.5%.

关 键 词：红叶乌桕 组织培养 褐化现象 

分 类 号：S722.37[农业科学—林木遗传育种]