高效液相色谱法同时测定减肥类保健食品中的6种成分  

Simultaneous Determination of Six Ingredients in Slimming Foods by High Performance Liquid Chromatography

作　　者：钟艳琴 史莹[2] 王丹琪[1] 肖双[1] ZHONG Yanqin;SHI Ying;WANG Danqi;XIAO Shuang(Changsha Medical University,Changsha 410205;Healthy Food Evaluation Research Center,West China School of Public Health,Sichuan University,Chengdu 610041)

机构地区：[1]长沙医学院公共卫生学院,湖南长沙410205 [2]四川省食品安全监测与风险评估重点实验室,四川大学华西公共卫生学院,四川成都610041

出　　处：《分析科学学报》2020年第3期449-452,共4页Journal of Analytical Science

基　　金：2017年度湖南省教育厅科学研究项目(17C0154)。

摘　　要：建立了一种同时测定减肥类保健食品中的荷叶碱,以及5种大黄蒽醌类化合物共6种成分的高效液相色谱测定方法。样品采用70%乙醇-水溶液超声提取30 min,离心后取上清液进样分析。菲罗门-Luna C18柱(250 mm×4.6 mm,5μm)为分离柱,流动相为甲醇(A)和0.02%H 3PO 4溶液,梯度洗脱,254 nm作为检测波长。6种物质的线性相关系数在0.9992~0.9999范围内。各种目标化合物的方法日内精密度为0.79%~6.3%,日间精密度为0.90%~8.1%,加标回收率在62.5%~115.0%之间。该方法已成功应用于8种样品中6种目标物的测定。A high performance liquid chromatography(HPLC)method was developed for simultaneous determination of six functional components in slimming foods.The components were ultrasonically extracted with 70%ethanol aqueous solution and then centrifuged,and finally the supernatant was injected into HPLC for analysis.A Phenomenex Luna C18 column(250 mm×4.6 mm,5μm)was applied for chromatographic separation.The mobile phase consisted of methanol and aqueous solution of 0.02%(v/v)phosphoric acid with gradient elution.The column temperature was kept at 25 o C and the detection wavelengths were set at 254 nm for different components.Good linearities(R2>0.9992)for all components were obtained with the intra-and inter-day precision(n=6)ranging from 0.79%to 6.3%and 0.90%to 8.1%,respectively.The average recoveries from the spiked samples varied from 62.5%to 115.0%.The proposed method has been successfully applied to the determination of the target components in eight slimming foods with satisfactory results,which can provide some feasible reference for the analysis of functional components in slimming foods.

关 键 词：高效液相色谱法 有效成分 减肥类保健食品 

分 类 号：O657.7[理学—分析化学]