氯雷他定对氧化型低密度脂蛋白诱导人血管内皮细胞黏附和炎症反应的影响  

Effects of loratadine on human aortic endothelial cell adhesion and inflammation induced by oxidized low density lipoprotein

作　　者：李贵琦[1] 周玉阳[2] 法宪恩[2] 万大国[1] 刘雷[2] 郭臣 LI Guiqi;ZHOU Yuyang;FA Xianen;WAN Daguo;LIU Lei;GUO Chen(Department of Cardiovascular Medicine,the Second Affiliated Hospital of Zhengzhou University,Zhengzhou 450014,Henan Province,China;Department of Cardiovascular Surgery,the Second Affiliated Hospital of Zhengzhou University,Zhengzhou 450014,Henan Province,China)

机构地区：[1]郑州大学第二附属医院心血管内科,河南郑州450014 [2]郑州大学第二附属医院心血管外科,河南郑州450014

出　　处：《新乡医学院学报》2020年第7期606-610,共5页Journal of Xinxiang Medical University

基　　金：河南省医学科技攻关计划项目(编号:2018030163);河南省高等学校重点科研项目指导计划(编号:19B320044)。

摘　　要：目的探讨氯雷他定对氧化型低密度脂蛋白(ox-LDL)诱导的人血管内皮细胞黏附和炎症反应的影响。方法将培养的人体主动脉内皮细胞(HAECs)分为空白对照组、ox-LDL组、氯雷他定组,空白对照组细胞未做任何处理,ox-LDL组细胞采用10 mg·L^-1 ox-LDL处理24 h,氯雷他定组细胞采用10 mg·L^-1 ox-LDL1 mL和100μmol氯雷他定处理24 h。将3组细胞与钙黄绿素染色后的人单核细胞THP-1共同培养2 h,荧光显微镜下观察THP-1黏附情况,计算THP-1黏附率;采用Western blot法检测3组HAECs中血管细胞黏附分子-1(VCAM-1)、E-选择素(E-selectin)蛋白的表达,采用实时荧光定量聚合酶链反应检测3组HAECs中肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)-6和IL-8 mRNA表达。结果空白对照组、ox-LDL组和氯雷他定组HAECs的单核细胞黏附率分别为(23.00±8.46)%、(50.83±11.02)%、(36.67±7.97)%;ox-LDL组HAECs的单核细胞黏附率显著高于空白对照组(P<0.05),氯雷他定组HAECs的单核细胞黏附率显著低于ox-LDL组(P<0.05)。ox-LDL组HAECs中VCAM-1、E-selectin蛋白的相对表达量显著高于空白对照组(P<0.05),氯雷他定组HAECs中VCAM-1、E-selectin蛋白的相对表达量显著低于ox-LDL组(P<0.05)。ox-LDL组HAECs中TNF-α、IL-6、IL-8 mRNA相对表达量显著高于空白对照组(P<0.05),氯雷他定组HAECs中TNF-α、IL-6和IL-8 mRNA相对表达量显著低于ox-LDL组(P<0.05)。结论ox-LDL能够诱导HAECs黏附分子VCAM-1和E-selectin及炎症因子TNF-α、IL-6和IL-8的表达,而氯雷他定能抑制ox-LDL诱导的细胞黏附和炎症反应。Objective To investigate the effect of loratadine on human aortic endothelial cell adhesion and inflammation induced by oxidized low density lipoprotein(ox-LDL).Methods The cultured human aortic endothelial cells(HAECs)were divided into the blank control group,ox-LDL group and loratadine group.The cells in the blank control group were not given any intervention.The cells in the ox-LDL group were treated with 1 mL ox-LDL(10 mg·L^-1)for 24 hours.The cells in the loratadine group were treated with 1 mL ox-LDL(10 mg·L^-1)and 100μmol loratadine for 24 hours.The cells in the three groups were co cultured with monocytes THP-1 stained with calcein for 2 hours.The monocyte adhesion was observed under fluorescence microscope,and the monocyte adhesion rate was calculated.The expressions of vascular-cell adhesion molecule-1(VCAM-1)and E-selectin protein in HAECs of three groups were detected by Western blot method.The mRNA expressions of tumor necrosis factor-α(TNF-α),interleukin(IL)-6 and IL-8 in HAECs of three groups were detected by real-time fluorescent quantitative polymerase chain reaction.Results The monocyte adhesion rate of HAECs in the blank control group,ox-LDL group and loratadine group was(23.00±8.46)%,(50.83±11.02)%and(36.67±7.97)%,respectively;the monocyte adhesion rate of HAECs in the ox-LDL group was significantly higher than that in the blank control group(P<0.05),and the monocyte adhesion rate of HAECs in the loratadine group was significantly lower than that in the ox-LDL group(P<0.05).The relative expression of VCAM-1 protein and E-selectin protein in HAECs of the ox-LDL group was significantly higher than that of the blank control group(P<0.05).The relative expression of VCAM-1 protein and E-selectin protein in HAECs of the loratadine group was significantly lower than that of the ox-LDL group(P<0.05).The relative expression of TNF-α,IL-6 and IL-8 mRNA in HAECs of the ox-LDL group was significantly higher than that of the blank control group(P<0.05);and the relative expression of TNF-α,IL-6 an

关 键 词：氧化型低密度脂蛋白 动脉粥样硬化 血管内皮细胞 炎症反应 氯雷他定 

分 类 号：R543[医药卫生—心血管疾病]