肌腱蛋白C在百草枯中毒肺损伤中的作用  

Tenascin-C participates in the process of paraquat-induced lung injury

作　　者：张迪[1] 刘倩倩 彭瑾瑾 刘志[1] 刘晓伟[1] 刘伟[1] ZHANG Di;LIU Qianqian;PENG Jinjin;LIU Zhi;LIU Xiaowei;LIU Wei(Emergency Department,The First Hospital,China Medical University,Shenyang 110001,China)

机构地区：[1]中国医科大学附属第一医院急诊科,沈阳110001

出　　处：《中国医科大学学报》2020年第8期711-715,共5页Journal of China Medical University

基　　金：国家自然科学基金(81571882,81772053)。

摘　　要：目的探讨肌腱蛋白C (TNC)在百草枯(PQ)中毒小鼠肺损伤中的表达及作用。方法 C57BL/6雄性小鼠经腹腔注射30 mg/kg PQ建立动物模型,对照组为同等剂量生理盐水腹腔注射。分别于接触PQ后2 d、7 d、14 d和28 d,麻醉后处死小鼠取出肺组织,苏木精-伊红染色法(HE)和胶原染色证明模型成功。逆转录定量聚合酶链式反应(RT-qPCR)、Western blotting和免疫组化(IHC)方法检测TNC基因和蛋白在小鼠肺组织中随时间的表达规律及定位,Western blotting检测核因子-κB p65 (NF-κB p65),α-平滑肌肌动蛋白(α-SMA)和转化生长因子-β1 (TGF-β1)在肺组织中随时间的表达规律。结果 TNC蛋白表达在接触PQ后2 d升高,7 d达高峰,之后表达降低,在14 d和28 d与对照组比较无统计学差异。TNC基因表达高峰提前到2 d,之后降低,在14 d和28d表达与对照组比较无统计学差异。IHC染色发现,TNC主要分布于损伤的肺泡间质区。NF-κB p65在接触PQ后2 d出现表达峰值,α-SMA在接触后14 d和28 d高表达,TGF-β1高表达贯穿整个实验始终(P <0.05)。结论首次发现TNC在PQ中毒诱发肺损伤过程中的表达及表达规律,提示TNC参与了此过程并发挥重要作用。Objective To analyze the role of tenascin-C(TNC) in paraquat(PQ)-induced lung injury. Methods Male C57 BL/6 mice(6 to 8 weeks old) were intraperitoneally injected with 30 mg/kg PQ and sacrificed on days 2,7,14,and 28 after PQ administration. We measured the expression levels of TNC protein in lung tissues of mice at all the time points when the mice were sacrificed by performing RT-qPCR,western blotting,and immunohistochemistry experiments. The protein expression levels of NF-κBp65,TGF-β1,and α-SMA in lung tissues were also measured. Results TNC protein and mRNA expression levels increased in lung tissues of C57 BL/6 mice after PQ administration. Furthermore,TNC protein expression levels peaked on day 7 after PQ exposure,which was much earlier than the time point at which TGF-β1 levels were observed to have peaked. TNC was mostly detected in the extracellular matrix of the thickened alveolar septum,especially at the sites of injury. TGF-β1 showed strong expression levels during the entire duration of the experiment. NF-κBp65 showed increased expression levels only on days 2 and 7. α-SMA protein levels increased significantly during later stages of lung injury at days 14 and 28. Conclusion TNC participates in the progression of lung injury induced by PQ poisoning.

关 键 词：肌腱蛋白C 百草枯 中毒 肺损伤 

分 类 号：R595.4[医药卫生—内科学]