表面电荷的调控对钛表面纤维连接蛋白吸附能力影响的研究  

Effect of Regulation of Surface Charge on Titanium Surfaces for Fibronectin Adsorption

作　　者：丁祥龙 李少冰 郭泽鸿 卢海宾 赖春花 吴静漪 高岩 王敬旭[2] 徐淑兰 Ding Xianglong;Li Shaobing;Guo Zehong;Lu Haibin;Lai Chunhua;Wu Jinyi;Gao Yan;Wang Jingxu;Xu Shulan(Center of Oral Implantology,Stomatological Hospital,Southern Medical University,Guangzhou 510280,China;Stomatology Department of the First Affiliated Hospital of Guangzhou Medical University,Guangzhou 510120,China)

机构地区：[1]南方医科大学口腔医院种植中心,广东广州510280 [2]广州医科大学第一附属医院口腔科,广东广州510120

出　　处：《稀有金属材料与工程》2020年第7期2431-2435,共5页Rare Metal Materials and Engineering

基　　金：国家自然科学基金(81600900);南方医科大学培育基金(PY2018016);南方医科大学口腔医院培育基金(PY2018N089)。

摘　　要：通过对钛片表面电荷的调控来改变纯钛表面的蛋白吸附能力,改善其生物学性能。将大颗粒喷砂酸蚀的样本分别浸泡在不同的溶液中,分别观察检测不同样本表面形貌及表面元素构成,各组样本的表面粗糙度,以及各组样本表面的电荷状况。最后检测各组样本的纤维连接蛋白吸附能力。结果表明,各组样本表面具有相似的表面形貌,不同组表面因为浸泡的液体不同其表面元素各不相同;各组样本的表面粗糙度无显著差异;而浸泡在氯化钙溶液中的钛片具有最高蛋白吸附能力(P<0.01),而对照组大颗粒喷砂酸蚀组吸附能力最弱。将钛片浸泡在含有二价钙离子的氯化钙溶液中可以改变钛片表面的电荷状态,提升蛋白吸附能力,从而改善其生物学性能。The fibronectin adsorptive capacity of pure titanium surfaces was investigated to improve its biological properties by regulating its surface charge.The different surfaces of titanium sheet were obtained by sandblasting with large grit and acid-etching(SLA),and then immersing in different solutions.Surface morphology,elemental composition,roughness,and surface charge status were studied,and fibronectin adsorption assay was performed for comparative analysis.The results show that the surface morphology of each group is similar,but the surface elements of the different groups are different because of immersion in different liquids.There is no significant difference in the surface roughness of each group.Titanium sheets immersed in calcium chloride solution have the highest protein adsorption capacity(P<0.01),whereas the control group has the lowest adsorption capacity.Immersion of titanium sheets in calcium chloride solution containing divalent calcium ions can change their surface charge,enhance their protein adsorption capacity,and thus improve their biological properties.

关 键 词：表面电荷 蛋白 种植体 细胞 

分 类 号：TG146.23[一般工业技术—材料科学与工程] TB383[金属学及工艺—金属材料]