无透镜全息显微细胞成像  

Cell imaging by holographic lens-free microscopy

作　　者：王雪 刘虹遥[1] 路鑫超 孙旭晴[1] 叶一霏 黄成军 WANG Xue;LIU Hong-yao;LU Xin-chao;SUN Xu-qing;YE Yi-fei;HUANG Cheng-jun(Institute of Microelectronics of the Chinese Academy of Sciences, Beijing 100029, China;University of Chinese Academy of Sciences, Beijing 100049, China)

机构地区：[1]中国科学院微电子研究所,北京100029 [2]中国科学院大学,北京100049 [3]中国科学院微电子研究所健康电子中心,北京100029 [4]中国科学院微电子研究所健康电子研发中心,北京100029

出　　处：《光学精密工程》2020年第8期1644-1650,共7页Optics and Precision Engineering

基　　金：国家重点研发计划资助项目(No.2018YFC2001100);北京市自然科学基金资助项目(No.4192063,4182073)。

摘　　要：为了同时满足较大的视场和较高分辨率的需求,开发了一套全息无透镜显微成像系统和配套算法,实现对微米级样品的无透镜显微成像。搭建了一套由LED光源、针孔、被测样品与CMOS图像传感器组成的全息无透镜显微成像系统,并对针孔直径、成像面尺寸、光源到样品的距离,以及样品面到CMOS图像传感器的距离进行了优化。其次,开发了从系统采集的全息图中恢复样品图像的角谱法算法。最后,使用该成像系统和配套算法,分别对具有微米级结构分辨率测试靶,和肺癌细胞悬浮液进行了显微成像。该全息无透镜显微成像系统的分辨率为4.4μm,成像视场尺寸为5.7 mm×4.3 mm,实现了微米级结构和肺癌细胞较清晰的显微成像。全息无透镜显微成像系统结构简单、无像差干扰,可以实现大视场下较高分辨率的显微成像。This study develops a lens-free microscope system and an algorithm to realize microscopic imaging with a relatively large field of view and high resolution.The proposed system consists of an LED light source,a pinhole,a sample holder,and a CMOS imager.The system parameters,such as the pinhole diameter,size of the imager,distance between the LED and sample,and distance between the sample and imager were studied and optimized.Furthermore,an angular spectrum method was developed for recovering sample images from holograms captured by the CMOS imager.Finally,the developed system was employed to demonstrate lens-free microscopic imaging of a micropatterned testing chip with a standard resolution in addition to that of lung cancer cells in suspension.The developed holographic lens-free microscopic system has a resolution of 4.4μm and an imaging field of view up to 5.7 mm×4.3 mm;furthermore,it is capable of imaging the micropatterns in the test chip as well as individual lung cancer cells.Thus,the proposed holographic lens-free microscopic system demonstrated microscopic imaging with a relatively large field of view and high resolution;moreover,it exhibited the advantages of a simple structure and the avoidance of aberration interference.

关 键 词：无透镜成像 全息图像重建 细胞成像 大视场 

分 类 号：O436.1[机械工程—光学工程] Q336[理学—光学]