新冠恢复期血浆拟捐献者健康评估及抗体水平分析  

Analysis of Health Status and Antibody Level of COVID-19 Convalescent Plasma Donors

作　　者：谢毓滨[1] 邹彬彬[1] 陈东 阳智芬 谭智文 王清[1] 旷开其[1] 晏永和 XIE Yu-bin;ZOU Bin-bin;CHEN Dong(Changsha Blood Center,Changsha,Hunan 410001)

机构地区：[1]长沙血液中心,410001 [2]长沙市第一医院

出　　处：《临床输血与检验》2020年第4期343-348,共6页Journal of Clinical Transfusion and Laboratory Medicine

基　　金：长沙市临床输血研究重点专科专项经费资助。

摘　　要：目的分析新冠肺炎康复者健康状况和抗体水平,为恢复期血浆采集和治疗选择合适的捐献者。方法收集2020年2月7日~3月8日长沙市新冠肺炎康复者出院时相关体检数据,对有意愿参加恢复期血浆捐献的康复者进行健康状况评估,评估合格者进行抗体检测。采用ELISA方法检测新型冠状病毒IgM、IgG抗体及总抗体,固相红细胞粘附法筛查血小板抗体,凝集法检测红细胞直接抗体及不规则抗体。结果15名拟捐献者平均年龄32岁,平均住院天数16.5 d,样本采集时距出院平均17.9 d。15名拟捐献者新冠核酸检测均呈阴性,有1名HBsAg阳性不符合捐献条件。15名拟捐献者直抗、不规则抗体筛查均阴性,血小板抗体筛查均呈非特异性阳性。20%(3/15)为IgM阴性,53.33%(8/15)IgG抗体滴度大于160,60%(9/15)总抗体滴度大于320,46.67%(7/15)同时满足IgG抗体滴度大于160及总抗体滴度大于320。结论46.67%~60%的拟捐献者可以进行恢复期血浆的捐献。采集前进行新冠肺炎康复者健康评估和抗体水平的检测,对于选择合适的捐献者,保障恢复期血浆治疗效果,具有重要意义。Objective To analyze the novel coronavirus pneumonia convalescents'health status and antibody levels,and select suitable donors for convalescent plasma collection and treatment.Methods The relevant medical examination data of discharged patients diagnosed with novel coronavirus pneumonia in the First Hospital of Changsha from February 7 to March 8,2020,and health status of those who were willing to participate in convalescent plasma donation were evaluated.ELISA was used to detect novel coronavirus IgM,IgG antibody and total antibody,solid phase red cell adherence assay was used to screen platelet antibody,agglutination was used to detect and irregular red cell antibodies.Results The average age of 15 donors was 32 years old,the average length of hospital stay was 16.5 days,and the average sample collection time was 17.9 days before discharge.2019-nCoV RNA screening results of 15 donors were all negative and 1 HBsAg positive was not qualified for donation.All 15 donors were negative for direct antibody and irregular antibody screening,and nonspecific positive for platelet antibody screening.20%(3/15)was IgM negative,53.33%(8/15)IgG antibody titer was greater than 160,60%(9/15)total antibody titer was greater than 320,46.67%(7/15)met both IgG antibody titer greater than 160 and total antibody titer greater than 320.Conclusion 46.67%~60%of proposed donors was permitted to donate convalescent plasma.It is of great significance to conduct the novel coronavirus pneumonia convalescents'health assessment and antibody level screening before collection,in order to select suitable donors and ensure the convalescent plasma treatment effect.

关 键 词：新冠恢复期血浆 新冠病毒抗体 直抗 不规则抗体 血小板抗体 

分 类 号：R457[医药卫生—治疗学]