咽拭子擦拭检测皮肤疣人乳头瘤病毒分型的敏感性研究  

Study on sensitivity of pharyngeal swab sampling for HPVs genotyping of cutaneous warts

作　　者：明星 尹萌萌 李京 吕世超[1] MING Xing;YIN Meng-meng;LI Jing(Department of Dermatology,306th Clinical College of Peoples Liberation Army,Anhui Medical University,Beijing 100101,China)

机构地区：[1]安徽医科大学解放军第306临床学院皮肤科,北京100101

出　　处：《实用皮肤病学杂志》2020年第3期136-138,共3页Journal of Practical Dermatology

基　　金：首都临床特色应用研究(Z141107002514179)。

摘　　要：目的研究咽拭子擦拭皮肤疣表面检测人乳头瘤病毒(HPV)分型的敏感性,改进皮肤疣HPV分型检测的取材方法。方法招募50例皮肤疣患者,用被细胞保存液浸湿的咽拭子采样头用力擦拭靶疣表面10次,再用外科手术刀片刮除同一疣体的部分疣体组织,两组方法取材的样本均低温保存;采用聚合酶链反应(PCR)、基因测序和TA克隆技术检测两组样本的HPV型别,通过与刀片刮除法的HPV型检测结果比较,计算咽拭子擦拭法的敏感性。结果刀片刮除、咽拭子擦拭组的HPV型检出率分别为100%(50/50)和92%(46/50),咽拭子擦拭法的敏感性为92%。结论咽拭子擦拭法可作为检测皮肤疣HPV型的常规取材方法。Objective To study the sensitivity of pharyngeal swab sampling from wart surface for HPVs genotyping,so as to improve the sampling method for HPVs genotyping of cutaneous warts.Methods A total of 50 patients with cutaneous warts were recruited in the study,and the pharyngeal swab sampling head soaked in cell preservation solution was used to firmly rub the surface of the patients’target lesions for 10 times,meanwhile,part of the same wart tissues were scraped with surgical blade,and the samples were cryopreserved respectively,then HPVs genotyping was performed on all the samples by using PCR technology and TA cloning assay.Finally the sensitivity of pharyngeal swab sampling for HPVs genotyping was evaluated by comparison with sampling by scraping.Results HPVs DNA was detected in 100%(50/50)scraping samples and in 92%(46/50)samples got by pharyngeal swabs.Pharyngeal swab sampling showed identical typing results as the sampling method of scraping in 46 of the 50 cases(sensitivity:92%).Conclusion The method of pharyngeal swab sampling can be used in HPVs genotyping of cutaneous warts.

关 键 词：皮肤疣 咽拭子擦拭法 人乳头瘤病毒 敏感性 

分 类 号：R752.5[医药卫生—皮肤病学与性病学]