高脂高糖饮食致非酒精性脂肪性肝病的证候属性研究  

Determination of TCM Syndromes in Non-Alcoholic Fatty Liver Disease Induced by High Fat and High Sugar Diet

作　　者：孙燕[1] 吴梦瑶 高誉珊[1] 张淑静[1] 王敏[1] 周璐[1] 张鑫蕾 陈琳[1] 李宇航[1] SUN Yan;WU Meng-yao;GAO Yu-shan;ZHANG Shu-jing;WANG Min;ZHOU Lu;ZHANG Xin-lei;CHEN Lin;LI Yu-hang(Beijing University of CM,Beijing,China,100029)

机构地区：[1]北京中医药大学,北京100029

出　　处：《河南中医》2020年第8期1219-1226,共8页Henan Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81673868)。

摘　　要：目的:对高脂高糖饮食致非酒精性脂肪性肝病的证候属性研究。方法:将20只健康雄性SD大鼠按数字表法随机分为正常组与模型组,每组各10只。正常组给予维持饲料喂养,模型组给予高脂高糖饲料喂养,连续喂养16周。造模过程中动态观察两组大鼠的情志、舌色、皮毛、二便等宏观表征,记录每日饮食、饮水量及每周体质量。对大鼠进行旷场实验、糖水实验,并检测大鼠的大便含水量和内脏脂肪指数,建立计量化评分公式,确定模型大鼠的中医证候。结果:正常组大鼠在16周内整体情况良好,形体适中,反应灵敏,活动自如,抓取反抗程度适中,没有打架互殴情况出现,毛色光亮,毛质顺滑,眼睛色泽淡红,舌色淡红,纳食佳,大小便均正常。模型组大鼠出现了明显形体消瘦,摄食、饮水量明显减少,并伴有精神烦躁,打架互殴,多动,眼睛发赤,毛色枯黄,大便硬结,小便量少等异常表现。与正常组比较,模型组大鼠第1周旷场得分比较无统计学意义(P>0.05),第12周、第16周旷场得分均显著升高,与正常组比较,差异有统计学意义(P<0.05)。模型组大鼠快感缺乏度明显升高,与正常组比较,差异有统计学意义(P<0.05)。与正常组比较,模型组大鼠大便含水量显著降低,差异有统计学意义(P<0.05)。与正常组比较,模型组大鼠内脏脂肪指数显著升高,差异有统计学意义(P<0.05)。正常组仅有1只大鼠肝郁评分>1.5分,其余均<1.5分,模型组所有大鼠肝郁评分均>1.5分。正常组大鼠痰湿评分多为1分,上下浮动均未超过0.5分,其分值均<1.5分;模型组有2只大鼠痰湿得分>1.5分,其余均为1~1.5分。正常组大鼠的热结评分多维持在1分左右,上下浮动未超过0.5分,其分值均<1.5分,而模型组大鼠的分值明显>1.5分,均为1.8~1.9分。结论:通过模型动物的宏观表征观察、临床同质性比较以及证候评分,加之中医病理机制的分析,最终判定,采用Objective:To determine the TCM syndromes of non-alcoholic fatty liver disease(NAFLD)induced by high-fat and high sugar diet.Methods:A total of 20 healthy male SD rats were randomly divided into the normal group and the model group,with 10 rats in each group.The normal group was fed with maintenance feed,and the model group was fed with high fat and high sugar diet.Both groups were fed for 16 weeks.In the process of modeling,the emotion,tongue color,fur,stool and urine and other macroscopic characteristics of the two groups were dynamically observed,and the daily diet,drinking water amount and body weight of each week were recorded.Open field experiment and sugar water test were carried out on rats,and the water content of stool and visceral fat index were detected,and the quantitative score formula was established to determine the TCM syndromes of the model rats.The rats in the normal group were in good condition within 16 weeks,with moderate body shape,sensitive reaction,free movement,moderate resistance to grasping,no fighting,bright fur,light red eyes,light red tongue,good food intake,and normal urine and stool.The rats in the model group were obviously emaciated,the amount of food and drinking water decreased significantly,accompanied by irritability,fighting,hyperactivity,red eyes,withered and yellow hair,stool induration,and less urine volume.Results:Compared with the normal group,the open field scores of rats in the model group in the first week were not statistically significant(P>0.05),while the scores of open field after12 th week and 16 th week were significantly increased,and all the differences were statistically significant(P<0.05).In the model group,the lack of pleasure was significantly increased,and compared with the normal group,the difference was statistically significant(P<0.05).Compared with the normal group,the stool water content of the model group was significantly decreased,and the difference was statistically significant(P<0.05).Compared with the normal group,the visceral fat index of th

关 键 词：非酒精性脂肪性肝病 中医证候 肝郁热结证 高脂高糖饮食 大鼠 

分 类 号：R285.5[医药卫生—中药学]