Development of oligonucleotide probes for FISH karyotyping in Haynaldia villosa,a wild relative of common wheat  

作　　者：Jia Lei Jiawen Zhou Haojie Sun Wentao Wan Jin Xiao Chunxia Yuan Miroslava Karafiátová Jaroslav Dolezel Haiyan Wang Xiue Wang 

机构地区：[1]State Key Laboratory of Crop Genetics and Germplasm Enhancement,Cytogenetics Institute,Nanjing Agricultural University/JCIC-MCP,Nanjing 210095,Jiangsu,China [2]Institute of Experimental Botany,Czech Academy of Sciences,Centre of the Region Hana for Biotechnological and Agricultural Research,Šlechtitelů31,77900 Olomouc,Czech Republic

出　　处：《The Crop Journal》2020年第4期676-681,共6页作物学报（英文版）

基　　金：supported by the National Key Research and Development Program of China(2016YFD0102001);the National Natural Science Foundation of China(31571653,31771782,31201204,31501305);International Cooperation and Exchange Programme of the National Natural Science Foundation of China(31661143005);Introducing the Technique to Exploring the Genetic Germplasm Based on the Chromosome Sorting and Sequencing(2015-Z41);the Special Fund of Jiangsu Province for the Transformation of Scientific and Technological Achievements(BA2017138);supported by European Regional Development Fund Project“Plants as a Tool for Sustainable Global Development”(CZ.02.1.01/0.0/0.0/16_019/0000827)。

摘　　要：Haynaldia villosa is a wild relative of wheat and a valuable gene resource for wheat improvement.Owing to the limited number of probes available for fluorescence in situ hybridization(FISH),the resolution at which the karyotype of H.villosa can be characterized is poor,hampering accurate characterization of small segmental alien introgressions.We designed ten oligonucleotide probes using tandem repeats in DNA sequences derived from the short arm of H.villosa chromosome 6 V(6 VS).FISH with seven of them resulted in clear signals on H.villosa chromosomes.Using these,we constructed FISH karyotypes for H.villosa using oligo-6 VS-1 and oligo-6 VS-35 oligonucleotides and characterized the distribution of the two probes in five different H.villosa accessions.The new FISH probes can efficiently characterize H.villosa introgressions into wheat.

关 键 词：Chromosome identification Haynaldia villosa Oligonucleotide probes Tandem DNA repeats 

分 类 号：S512.1[农业科学—作物学]