Effects of extracts from Renshen(Radix Ginseng), Sanqi(Radix Notoginseng), and Chuanxiong(Rhizoma Chuanxiong) on F-actin in senescent microvascular endothelial cells  

作　　者：Wang Qiang Yang Jing Lei Yan Xiu Chengkui Huo Yanming Shi Hang 

机构地区：[1]Department of Cardiology,Wangjing Hospital,China Academy of Chinese Medical Sciences,Beijing 100102,China [2]Graduate School of Beijing University of Chinese Medicine,Beijing 100029,China [3]Experimental Research Center,China Academy of Chinese Medical Sciences,Beijing 100700,China

出　　处：《Journal of Traditional Chinese Medicine》2020年第3期376-385,共10页中医杂志（英文版）

基　　金：Supported by the National Natural Science Foundation of China(the Study on Intervention and Mechanism of Chinese Herbal Medicines for Nourishing Qi and Resolving Blood Stasis on Cytoskeletal Protein of Vascular Aging(No.81273976);the Mechanism of Exploration about Formula for Reinforcing Qi,Warming Yang,Activating Blood and Promoting Diuresis in the Prevention and Treatment of Heart Failure through mT ORC1-Mitophagy Pathway(No.8170-3865);Independent Research Program of the China Academy of Chinese Medical Sciences(the Study on Mechanism of Chinese Herbal Medicines for Nourishing Qi and Resolving Blood Stasis Delaying Vascular Aging(No.zz2013002);the Mechanism of Exploration about Formula for Reinforcing Qi,Warming Yang,Activating Blood and Promoting Diuresis in the Prevention and Treatment of Heart Failure through Nrf2-iron Death Pathway(No.zz13-YQ-034);Beijing Science and Technology Bureau(Clinical study about Formula for Reinforcing Qi,Warming Yang,Activating Blood and Promoting Diuresis to Improve the Survival Status of Patients with Chronic Heart Failure(No.Z171100001017108)。

摘　　要：OBJECTIVE: To investigate the effects of extracts from Renshen(Radix Ginseng), Sanqi(Radix Notoginseng), and Chuanxiong(Rhizoma Chuanxiong) on the endothelial actin cytoskeleton in senescent human cardiac microvascular endothelial cells(HCMECs), and to propose the possible mechanism underlying the actions.METHODS: Lentiviral mediated RNA interference was applied to a replicative senescent HCMEC model by knocking down heat shock protein 27(HSP27)gene. Cells were treated with extracts from Renshen(Radix Ginseng), Sanqi(Radix Notoginseng), and Chuanxiong(Rhizoma Chuanxiong) at final concentrations of 50, 100, and 200 mg/L, respectively and with 10 μM resveratrol for 48 h. Untreated cells were used as controls. Senescence was detected by senescence β-galactosidase staining and cell proliferation was analyzed by cell counting kit-8 assays.Secreted nitric oxide levels were detected by nitrate reductase. Morphological changes of F-actin and G-actin were observed by laser scanning confocal microscopy. Protein and gene expression of Factin and HSP27 was detected by western blotting.RESULTS: Compared with the control group, the proportion of senescent HSP27 shRNA cells treated with the extracts was decreased and their proliferation was increased. In the extract intervention group, F-actin around the cell periphery became irregular and jagged fractures formed gradually and then dissipated. Moreover, some dynamic actin stress fiber filaments appeared. The G-actin stretched to the cell periphery and punctate staining was scattered in the cytoplasm. In addition, the mean optical density value of F/G-actin was decreased significantly and the protein expression of F-actin was downregulated.CONCLUSION: The extracts delayed microvascular endothelial cell senescence by downregulating the expression of F-actin through HSP27.

关 键 词：Endothelial cells Aging Actin cytoskeleton HSP27 heat-shock proteins Renshen(Radix Ginseng) Sanqi(Radix Notoginseng) Chuanxiong(Rhizoma Chuanxiong) 

分 类 号：R285.5[医药卫生—中药学]