苦豆子主要生物碱含量表型性状的SSR标记关联分析  

Association analysis of phenotypic traits of alkaloid content in Sophora alopecuroides with SSR molecular markers

作　　者：李文娟 刘姣蓉 周玉梅[1,2] 洪园淑 刘萍 LI Wen-juan;LIU Jiao-rong;ZHOU Yu-mei;HONG Yuan-shu;LIU Ping(Key Laboratory of Modern Molecular Breeding for Dominant and Special Crops in Ningxia,Yinchuan 750021,China;College of Agronomy,Ningxia University,Yinchuan 750021,China)

机构地区：[1]宁夏优势特色作物现代分子育种重点实验室,宁夏银川750021 [2]宁夏大学农学院,宁夏银川750021

出　　处：《中国中药杂志》2020年第13期3104-3111,共8页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81760685)。

摘　　要：为深入研究并充分利用药用植物苦豆子Sophora alopecuroides,寻找与苦豆子生物碱含量相关联的分子标记。该研究利用SSR分子标记对23个苦豆子居群的遗传多样性和群体结构进行探讨,并在此基础上与主要生物碱含量的表型性状做关联分析。23个苦豆子居群P=40.10%,H=0.335 3,I=0.504 5,Gst=0.433 7,Nm=0.625 9,表明不同苦豆子居群间基因交流比较少,遗传分化大。UPGMA聚类结果显示来自甘肃、内蒙古、青海的苦豆子居群与来自宁夏的苦豆子居群亲缘关系较近,而来自新疆的苦豆子居群则与其他地区的苦豆子居群亲缘关系较远。群体结构分析将23个苦豆子居群分为2个亚群。关联分析表明13个SSR标记共26个位点分别与MA,OMA,SC,OSC含量4个表型性状相关联(P<0.005),单个关联标记位点的表型变异解释率(R^2)为36.45%~77.93%,其中高阈值(P<0.000 1)下与MA和OSC含量相关联的位点各有1个,其解释率均超过50%;此外,引物S29在P<0.005水平下共有7个位点与MA含量相关联。研究结果为苦豆子生物碱生物合成代谢途径中重要基因的发掘奠定基础。To further study and fully exploit the medicinal plant Sophora alopecuroides,the molecular markers related with the phenotypic traits of alkaloid content in S.alopecuroides should be detected.In this study,SSR molecular markers were used to analyze the genetic diversity and genetic structure of 23 S.alopecuroides populations,in combination with the association analysis between molecular markers and the alkaloid contents.The results showed that P,H,I,Gst and Nm values were 40.10%,0.3353,0.5045,0.4337 and 0.6259 respectively,in 23 S.alopecuroides populations.This indicated that there was less gene exchange and higher genetic differentiation among different S.alopecuroides populations.The results of SSR unweighted pair-group method with arithmetic means(UPGMA)cluster showed that the S.alopecuroides populations relationship from Xinjiang was far from the populations of other regions,but the populations of S.alopecuroides from Gansu,Inner Mongolia and Qinghai were closely relevant to those from Ningxia.The 23 populations were further divided into 2 genetic subpopulations by the population structure analysis.Through association analysis,a total of 26 loci in 13 SSR markers were found to be significantly associated(P<0.005)with the content of MA,OMA,SC and OSC,and the rate of explanation on the phenotype variance of related markers ranged from 36.45%to 77.93%.Among the locus,1 each were related with MA and OSC content at interpretation rate reached as high as 50%with high threshold(P<0.0001).These results could provide support for the discovery of important genes in the alkaloid biosynthetic and metabolic pathway of S.alopecuroides.

关 键 词：苦豆子 生物碱 SSR标记 群体结构 关联分析 

分 类 号：S567.239[农业科学—中草药栽培]