高尿酸血症大鼠氧化应激介导的“肠-肾”尿酸排泄研究  

Intestinal-renal uric acid excretion mediated by oxidative stress in hyperuricemic rats

作　　者：王雨[1] 林志健[1] 曲聪聪 邹丽娜 张冰[1] Wang Yu;Lin Zhijian;Qu Congcong;Zou Lina;Zhang Bing(School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 102488,China)

机构地区：[1]北京中医药大学中药学院,北京102488

出　　处：《北京中医药大学学报》2020年第4期310-316,共7页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(No.81673618)。

摘　　要：目的从尿酸排泄相关的"肠-肾"途径切入,在整体及器官水平上系统观察高血尿酸状态下机体氧化应激损伤。方法 10%果糖饮水建立高尿酸血症大鼠模型。动态检测大鼠血清尿酸(SUA)、活性氧(SROS)、丙二醛(SMDA)、超氧化物歧化酶(SSOD)、谷胱甘肽过氧化物酶(SGSH-PX)、过氧化氢酶(SCAT)和总抗氧化能力(ST-AOC)水平,以及粪便尿酸(FUA)和尿尿酸(UUA)含量。Pearson检验分析SUA、FUA、UUA与SROS的相关性。制作组织匀浆,检测肠丙二醛(IMDA)、肾丙二醛(RMDA)、肠超氧化物歧化酶(ISOD)、肾超氧化物歧化酶(RSOD)水平。结果实验第10、20、30天,模型组大鼠SUA、SROS、SMDA水平升高,ST-AOC水平降低,且SUA与SROS呈显著正相关(P<0.01)。实验第20、30天,模型组大鼠FUA、SSOD、SGSH-PX水平均降低;实验第30天,模型组大鼠UUA水平降低;FUA、UUA均与SROS显著负相关(P<0.05或P<0.01)。模型组大鼠肠道及肾组织中MDA水平均升高,肠道及肾组织中ISOD水平均降低。结论 10%高果糖诱导的大鼠高尿酸血症模型中机体氧化应激水平增加,该状态下肠-肾尿酸排泄障碍与氧化应激引起的肠-肾组织氧化损伤有关。Objective To observe the intestinal and renal uric acid excretion and oxidative stress condition in hyperuricemia from the organismic level in animals. Methods Hyperuricemic rat model was induced by 10% fructose drinking. The levels of serum uric acid(SUA), serum reactive oxygen species(SROS), serum malondialdehyde(SMDA), serum superoxide dismutase(SSOD), serum glutathion peroxidase(SGSH-PX), serum catalase(SCAT), serum total antioxidant capacity(ST-AOC) and fecal uric acid(FUA), urinary uric acid(UUA) were detected every ten days. The correlation among SUA, FUS, UUA and SROS was analyzed by Pearson correlation analysis. Tissues of the kidney and the intestinal were homogenated for measurement of renal/intestinal malondialdehyde and renal/intestinal superoxide dismutase. Results From the 10th to the 30th days, levels of SUA, SROS, SMDA in the model group was higher than that in normal group, whereas ST-AOC level was decreased significantly in the model group. A positive correlation between SUA and SROS was observed. From the 20th to the 30th days, levels of FUA, SSOD, and SGSH-PX were significantly decreased in the model group. The level of UUA was significantly decreased in the model group at the 30th day. There was no difference of SCAT levels between the two groups. The negative correlation between FUA and SROS as well as UUA and SROS were also observed. In addition, levels of IMDA and RMDA were obviously increased in the model group, whereas levels of ISOD and RSOD were significantly decreased. Conclusion Decreased urate excretion of intestinal-renal axis was mediated by the enhanced oxidative stress reaction in hyperuricemia induced by 10% fructose drinking.

关 键 词：高尿酸血症 肠-肾排泄途径 氧化应激 大鼠 

分 类 号：R285.5[医药卫生—中药学]