酪胺修饰石墨烯量子点检测血清中游离胆固醇  

Determination of Free Cholesterol in Serum by Tyramine Modified Graphene Quantum Dots

作　　者：马志莹 高雪 宋宇 刘秀英 励建荣 Ma Zhiying;Gao Xue;Song Yu;Liu Xiuying;Li Jianrong(Engineering and Technology Research Center of Food Preservation,Processing and Safety Control of Liaoning Province,Liaoning Provincial Key Laboratory of Food Quality Safety and Functional Food,College of Food Science and Engineering,Bohai University,Jinzhou,121013)

机构地区：[1]渤海大学食品科学与工程学院辽宁省食品安全重点实验室,生鲜农产品贮藏加工及安全控制技术国家地方联合工程研究中心,锦州121013

出　　处：《化学通报》2020年第7期659-664,共6页Chemistry

基　　金：国家自然科学基金项目(31501564)资助。

摘　　要：以柠檬酸为原料合成具有模拟过氧化氢酶活性的石墨烯量子点(GQDs),经酪胺修饰的GQDs(TYR-GQDs)可以将过氧化氢还原成羟基自由基,通过酚羟基之间的交联聚集,GQDs的荧光被猝灭。由于胆固醇氧化酶可以催化胆固醇氧化生成H2O2,因此,基于TYR-GQDs建立了一种检测胆固醇的荧光传感器。在pH=7.4的条件下,TYR-GQDs的荧光猝灭率与胆固醇浓度(2.67×10^-8~2.67×10^-3mol/L)的对数呈良好的线性关系,检出限为9.32×10^-9mol/L。干扰实验表明,该荧光传感器对胆固醇具有高度的选择性,可以用于人体血清中游离胆固醇的检测,加标回收率为96.55%~100.14%。In this study,graphene quantum dots(GQDs)with catalase activity were synthesized from citric acid.GQDs modified by tyramine(TYR-GQDs)can reduce hydrogen peroxide to hydroxyl radicals.GQDs aggregates through the cross-linking between phenolic hydroxyls,which leads to fluorescence quenching.Since cholesterol oxidase can catalyze the oxidation of cholesterol to produce H2O2,a fluorescence sensor for detecting cholesterol was established based on TYR-GQDs.The experimental results showed that under the condition of pH=7.4,the fluorescence quenching rate of TYR-GQDs exlibits a good linear relationship with the logarithm of cholesterol concentration(2.67×10^-8~2.67×10^-3 mol/L),and the detection limit is 9.32×10^-9 mol/L.The interference experiment showed that the fluorescent sensor has high selectivity for cholesterol and can be used for the detection of free cholesterol in human serum.The recovery of standard addition is 96.55%~100.14%.

关 键 词：TYR-GQDs H2O2 胆固醇 

分 类 号：R446.1[医药卫生—诊断学] O657.1[医药卫生—临床医学]