细菌磷酸转移酶系统(PTS)的组成与功能研究进展  

Research progress of the composition and function of bacterial phosphotransferase system

作　　者：刘倩钰 吴丽雯 牛建军[2] 赵西林[1] LIU Qian-Yu;WU Li-Wen;NIU Jian-Jun;ZHAO Xi-Lin(State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics,School of Public Health,Xiamen University,Xiamen,Fujian 361102,China;Zhongshan Hospital,School of Medicine,Xiamen University,Xiamen,Fujian 361004,China)

机构地区：[1]厦门大学公共卫生学院,分子疫苗学和分子诊断学国家重点实验室,福建厦门361102 [2]厦门大学附属中山医院,福建厦门361004

出　　处：《微生物学通报》2020年第7期2266-2277,共12页Microbiology China

基　　金：国家自然科学基金(81971905);福建省海洋经济发展补助资金项目(FJHJF-L-2019-4);分子疫苗学与分子诊断学国家重点实验室开放研究项目(SKLVD2018KF005)。

摘　　要：细菌磷酸烯醇丙酮酸(phosphoenolpyruvate,PEP)-磷酸转移酶系统(phosphotransferase system,PTS)广泛存在于细菌、真菌和一些古细菌中,但不存在于动植物中。PTS由酶I(EI)、组氨酸磷酸载体蛋白(HPr或NPr)和酶II复合物等磷酸转移酶组成,既具有催化转运功能,又具有非常广泛的调节功能。PTS主要是通过磷酸级联反应将各种糖及其衍生物进行磷酸化然后运输到胞内。其不仅参与碳、氮中心代谢,调节铁、钾稳态,调控某些病原体的毒力,还能介导应激反应。在这些不同的调节过程中,信号由PTS组分的磷酸化状态提供,而该磷酸化状态根据PTS底物的可用性和细胞代谢状态的变化而变化。本文对细菌中磷酸转移酶系统的组成和调控网络进行综述,以期为PTS的整体调控机制及其对细菌整体代谢影响的研究提供参考依据。The bacterial phosphoenolpyruvate(PEP)-phosphotransferase system(PTS)is widely found in bacteria,fungi and some archaea,but not in plants and animals.PTS is composed of phosphotransferases such as enzyme I(EI),histidine phosphate carrier protein(HPr or NPr),and enzyme II complex.It has both a catalytic transport function and a very extensive regulatory function.PTS mainly phosphorylates various sugars and their derivatives through a phosphate cascade and then transports them into the cell.It not only participates in the metabolism of carbon and nitrogen sources,regulates the homeostasis of iron and potassium,regulates the virulence of certain pathogens,but it also mediates stress responses.During these different regulatory processes,the signal is provided by the phosphorylation state of the PTS components,which changes according to the availability of the PTS substrate and the metabolic state of the cell.This article reviews the composition and regulatory network of phosphotransferase systems in bacteria,with a goal to provide a knowledge base for the study of the overall regulatory mechanism of the PTS and its effect on the overall metabolism of bacteria.

关 键 词：磷酸转移酶系统(PTS) 碳代谢 氮代谢 铁、钾调节 毒力 应激反应 

分 类 号：Q936[生物学—微生物学]