复方红根草片及其组方对多重耐药菌的抗菌活性及逆转耐药性的研究  

Study on the Antibacterial Activity and Reverse Drug Resistance of Compound Honggen Grass Tablet and Its Components against Multidrug Resistant Bacteria

作　　者：胡俊辉[1] 王莹[1] 赵静怡[2] 李建会[2] 肖旭[1] 姜艳平[1] 姚银辉[1] 宋红 HU Junhui;WANG Ying(The Affliated Hospital of Chengde Medical College,Hebei Chengde 067000,China)

机构地区：[1]承德医学院附属医院药学部,河北承德067000 [2]承德医学院,河北承德067000

出　　处：《河北医学》2020年第7期1217-1220,共4页Hebei Medicine

基　　金：河北省承德市科学技术研究与发展计划项目,(编号:201701A087)。

摘　　要：目的:复方红根草片及其组方中5种中药材对多重耐药菌的体外抗菌活性及逆转耐药性研究,为中药抗菌治疗提供依据.方法:收集住院病人痰液分离的耐药菌鲍曼不动杆菌和肺炎克雷伯菌,采用微量肉汤稀释法确定受试菌的MIC值,计算联合用药指数∑FIC以判断逆转耐药性与否.结果:体外抗菌活性试验示复方红根草片对多重耐药鲍曼不动杆菌无抑菌效果,对多重耐药肺炎克雷伯菌有抑菌效果.逆转耐药性试验示复方红根草片与亚胺培南、头孢哌酮舒巴坦及庆大霉素分别联合用药时,有协同作用,其组方中药材分别与亚胺培南、头孢哌酮舒巴坦、庆大霉素、头孢吡肟联用时,对多重耐药肺炎克雷伯菌有不同的协同作用.结论:复方红根草片及其组方5为中药材对多重耐药肺炎克雷伯菌的抗菌活性及逆转耐药性均有一定效果.Objective:To provide the basis for the antibacterial treatment of traditional Chinese medicine,the in vitro antibacterial activity and reverse drug resistance of 5 kinds of Chinese herbal medicines in compound honggencao tablet and its formulation were studied.Methods:The mic value of the drug-resistant bacteria,Acinetobacter baumannii and Klebsiella pneumoniae,isolated from the sputum of the inpatients,was determined by the method of broth dilution,and the index of combined use∑FIC was calculated to judge whether the drug resistance was reversed or not.Results:In vitro antibacterial activity test showed that compound Honggen tablet had no antibacterial effect on Acinetobacter baumannii and had antibacterial effect on Klebsiella pneumoniae.The reverse drug resistance test showed that the compound honggencao tablet had synergistic effect with imipenem,cefoperazone sulbactam and gentamycin respectively.The Chinese herbal medicines with imipenem,cefoperazone sulbactam,gentamycin and cefepime respectively had different synergistic effect on Klebsiella pneumoniae.Conclusion:Compound honggencao tablet and its compound 5 are Chinese herbal medicines,which have certain effect on antibacterial activity and reverse drug resistance of multi drug resistant Klebsiella pneumoniae.

关 键 词：复方红根草片 体外抗菌 多重耐药菌 

分 类 号：R285[医药卫生—中药学]