濒危植物领春木天然群体遗传多样性的SCoT分析  

Genetic Diversity of Natural Population in an Endangered Plant Species Euptelea pleiosperma Hook.f.et Thoms.Based on SCoT Markers

作　　者：吴琼[1] 张峰[2] 张谨华[1] 武玉珍[1] WU Qiong;ZHANG Feng;ZHANG Jinhua;WU Yuzhen(College of Biological Science and Technology,Jinzhong University,Jinzhong,Shanxi 030619;Institute of Loess Plateau,Shanxi University,Taiyuan,Shanxi 030006)

机构地区：[1]晋中学院生物科学与技术学院,山西晋中030619 [2]山西大学黄土高原研究所,山西太原030006

出　　处：《北方园艺》2020年第13期77-82,共6页Northern Horticulture

基　　金：山西省自然科学基金资助项目(2012011034-3,201701D121083);晋中学院“1331”工程创客团队资助项目(Jzxycktd2017009)。

摘　　要：以领春木天然群体为试材,采用SCoT分子标记技术,研究了山西中条山领春木天然群体的遗传多样性,以期为领春木种质资源的评价和保护提供分子生物学依据。结果表明:23条SCoT共扩增出108条,其中多态性条带80条,多态性比例为74.07%;观察等位基因数平均为1.71,有效等位基因数平均为1.50,Nei′s基因多样性平均为0.2817,香农信息指数平均为0.4117;38份供试材料的平均相似系数为0.855。这说明38份领春木种质资源的遗传多样性不高,遗传基础相对较为狭窄。38 germplasm resources were studied by SCoT molecular markers,in order to estimate genetic diversity of Euptelea pleiosperma in Zhongtiaoshan Mount of Shanxi Province,provide molecular basis for the evaluation and protection of it.The results showed that 108 bands were amplified by 23 SCoT primers,including 80 polymorphic bands,and the polymorphism rate was 74.07%.The average of observed number of alleles,effective number of alleles,Nei′s gene diversity,Shannon information index,and similarity coefficient were 1.71,1.50,0.2817,0.4117 and 0.855.It indicated that the genetic diversity occurred within Euptelea pleiosperma maxim was poor,and the genetic basis was narrow.

关 键 词：濒危植物 SCoT标记 领春木 天然群体 遗传多样性 

分 类 号：S792.99[农业科学—林木遗传育种]