麝香乌龙丸对S1P诱导血管内皮细胞通透性的调控作用  

Regulation of Shexiangwulong pill on permeability of vascular endothelial cells induced by S1P

作　　者：马炜秀 王志文 袁强 张爱国 曹颖 Ma Weixiu;Wang Zhiwen;Yuan Qiang;Zhang Aiguo;Cao Ying(North China University of Science and Technology, Tangshan 063210,China)

机构地区：[1]华北理工大学中医学院,河北唐山063210 [2]华北理工大学附属医院中西医结合科,河北唐山063000

出　　处：《疑难病杂志》2020年第8期839-844,共6页Chinese Journal of Difficult and Complicated Cases

基　　金：河北省自然科学基金项目(H2017209157);2015年政府资助省级临床医学优秀人才基金项目(361036);华北理工大学博士科研启动项目(35755599)。

摘　　要：目的通过1-磷酸鞘氨醇(S1P)诱导人脐静脉内皮细胞(HUVEC),观察麝香乌龙丸提取物对HUVEC通透性的调控作用,及其保护血管屏障、减轻类风湿性关节炎(RA)滑膜炎性反应的可能机制。方法2018年8月—2019年11月于华北理工大学中医学院实验室进行实验。以S1P 10μmol/L诱导HUVEC细胞建立血管内皮高通透性模型。将HUVEC分为对照组、S1P诱导组及麝香乌龙丸提取物组(药物组),后2组分别给予S1P 10μmol/L、S1P 10μmol/L+麝香乌龙丸提取物干预。比较3组HUVEC通透性变化及连接蛋白的表达。结果S1P诱导组血管内皮通透性较对照组增高(t/P=9.180/0.001),药物组血管内皮通透性较S1P诱导组降低(t/P=4.206/0.014)。与对照组比较,S1P诱导组ZO-1、claudin-5、occludin蛋白表达减少(t/P=7.393/0.002、15.437/0.000、15.808/0.000),与S1P诱导组比较,药物组3种蛋白表达均增加(t/P=6.586/0.003、3.554/0.024、2.971/0.041)。结论麝香乌龙丸提取物可以通过上调ZO-1、claudin-5及occludin表达,对高浓度S1P诱导的HUVEC通透性增加起到明显抑制作用。Objective By inducing human umbilical vein endothelial cells(HUVEC)with sphingosine 1 phosphate(S1P),the regulation of Shexiangwulong Pill extract on HUVEC permeability was observed,and the possible mechanism of protecting blood vessel barrier and alleviating synovitis reaction of rheumatoid arthritis(RA)was discussed.Method From August 2018 to November 2019,the experiment was conducted in the laboratory of College of traditional Chinese medicine,North China University of technology.HUVEC cells were induced by S1P 10μmol/L to establish a high permeability model of vascular endothelium.HUVEC was divided into control group,S1P induction group and Shexiangwulong Pill extract group(drug group).The latter two groups were given S1P 10μmol/L,S1P 10μmol/L+Shexiangwulong Pill extract intervention respectively.The permeability of HUVEC and the expression of connexin in three groups were compared.Result Compared with the control group,the vascular endothelial permeability in S1P group was higher(t/P=9.180/0.001),while that in drug group was lower(t/P=4.206/0.014).Compared with the control group,the expression of Zo-1,claudin-5 and occludin in the S1P induced group decreased(t/P=7.393/0.002,15.437/0.000,15.808/0.000).Compared with the S1P induced group,the expression of three proteins in the drug group increased(t/P=6.586/0.003,3.554/0.024,2.971/0.041).Conclusion The extract of Shexiangwulong Pill can significantly inhibit the increase of HUVEC permeability induced by high concentration of S1P by up regulating the expression of Zo-1,claudin-5 and occludin.

关 键 词：麝香乌龙丸 类风湿性关节炎 人脐静脉内皮细胞 血管内皮细胞通透性 

分 类 号：R28[医药卫生—中药学]