小豆蔻明对冻融精子运动参数的影响  

The influence of cardamonin on motion parameters of human sperm suffering freezing thawing process

作　　者：曾凡湘[1] 牛培广[1] 林典梁[1] 王航伟[1] 陈钢鑫 刘颖[1] 曾晓萍 吴安鹏 李逸伟 史道华[1] ZENG Fanxiang;NIU Peiguang;LIN Dianliang;WANG Hangwei;CHEN Gangxin;LIU Ying;ZENG Xiaoping;WU Anpeng;Li Yiwei;SHI Daohua(Fujian Maternity and Child Health Care Hospital, Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian Province, 350001)

机构地区：[1]福建省妇幼保健院,福建医科大学附属医院,福州350001

出　　处：《中国计划生育学杂志》2020年第5期641-644,共4页Chinese Journal of Family Planning

基　　金：福建省自然科学基金资助项目(2017J01239);福建省卫生和计划生育委员会青年科研课题资助(2014-1-17);福建省卫生健康青年科研课题资助(2019-2-24)。

摘　　要：目的:通过观察小豆蔻明(CAR)对冻融精子运动参数的影响,探讨CAR对精子的保护作用。方法:收集成年男性正常精液(25份)体外处理后进行冷冻复苏,精子悬液与冷冻保护剂体积比为1:1,分为6组:冷冻前组、冻融组、保护剂组和保护剂分别加低、中、高浓度CAR(0.5×10-6、1.0×10-6、2.0×10-6mol/L)各组;比较各组前向运动精子(PR)率、精子总活动率、曲线速率(VCL),直线速率(VSL)、平均路径速率(VAP)、平均角位移(MAD)和侧摆幅度(ALH)。结果:冻融组PR、总活动率、VCL、VSL、VAP、MAD和ALH均低于冷冻前组(P<0.05)。保护剂组PR、总活动率、VSL均高于冻融组(P<0.05)。冷冻保护剂组和CAR低、中、高浓度组PR有统计学差异(F=5.07,P=0.030);高浓度CAR组PR为(47.2±0.6)%,高于保护剂组(41.7±1.7)%(LSD-t=-5.47,P=0.005)。结论:CAR联合冷冻保护剂能一定程度上改善精子前向运动能力。Objective:To evaluate the protective effects of cardamonin(CAR)on injury of human sperm that suffered freezing-thawing process,and to explore the development and improvement of novel sperm cryoprotectant.Methods:25 semen samples were collected from healthy men and were pretreated in vitro before cryopreservation.The ratio of sperm suspension volume to cryopreservation agent volume was 1:1.The semen samples was divided into 6 groups,which included pre-cryopreservation group,freeze-thaw group,cryopreservation group,and protectant group with low concentration of CAR(0.5×10-6mol/L),protectant group with medium concentration of CAR(1.0×10-6 mol/L),and protectant group with high concentration of CAR(2.0×10-6 mol/L).The percentage of forward motility sperm(PR),the percentage of sperm overall motility(Motility),curvilinear velocity(VCL),velocity of straight line(VSL),average path velocity(VAP),mean angular displacement(MAD)and amplitude of lateral head displacement(ALH)were compared among the six groups.Results:Compared with those of the before cryopreservation group,the difference of all parameters of the cryopreservation group including PR,Motility,VCL,VAP,MAD and ALH had statistically significant different(P<0.05).The parameters including PR,Motility and VSL of semen in the cryoprotectant group were all statistical higher than those of semen in the cryopreservation group(P<0.05).The parameters of semen in the cryoprotectant group had significant different to those of semen in the protectant group with low,medium,or high concentration of CAR(F=5.07,P=0.030),and the PR of semen in the protectant group with high concentration of CAR was 47.2±0.6%,which was significant higher that(41.7±1.7%)of semen in the cryoprotectant group(LSD t=5.47,P=0.005).Conclusion:CAR combined with cryoprotectant can improve the forward motility of human sperm after suffered cryopreservation.

关 键 词：小豆蔻明 冷冻复苏 精子 损伤 保护 

分 类 号：R69[医药卫生—泌尿科学]