精子DNA碎片指数对体外受精-胚胎移植结局的影响  

Influence of sperm DNA fragment index on the outcome of in vitro fertilization embryo transfer

作　　者：孙园园[1] 李森 陈南侨[1] 曾小海[1] SUN Yuanyuan;LI Sen;CHEN Nanqiao;ZENG Xiaohai(The Second People's Hospital of Guangdong Province, Guangzhou, 510310)

机构地区：[1]广东省第二人民医院,广州510310

出　　处：《中国计划生育学杂志》2020年第5期718-721,共4页Chinese Journal of Family Planning

摘　　要：目的:探究精子DNA碎片指数(DFI)对体外受精-胚胎移植(IVF-ET)结局的影响。方法:选取2015年9月-2018年9月本院生殖医学中心IVF-ET助孕初筛患者486对,精子染色质结构分析法联合流式细胞术检测患者精子DAN碎片,根据DFI分为DFI≤15%组、15%<DFI≤30%组、DFI>30%组,观察IVF-ET过程并行胚胎培养。结果:DFI不同的各组患者禁欲时间、精液体积、精子浓度、正常受精率、卵裂率、生化妊娠率、临床妊娠率比较无差异(P>0.05)。向前精子活力、正常精子形态率、优质胚胎率、优质胚囊率、囊胚形成率、生化妊娠率、临床妊娠率DFI>30%组低于另外两组(P<0.05),DFI>30%组生化妊娠率、临床妊娠率低于DFI≤15%组(P<0.05)。结论:DFI升高会导致精子前向活动力降低,影响精液质量,降低优质胚胎率、优质胚囊率、囊胚形成率,影响妊娠结局。Objective:To study the influence of DNA fragmentation index(DFI)on the outcome of in vitro fertilization embryo transfer(IVF-ET).Methods:486 pairs who accepted IVF-ET were selected in this study from September 2015 to September 2018.Sperm chromatin structure assay(SCSA)combined with flow cytometry was used to detect the Dan DNA fragments of spermatozoa,and these included pairs were divided into group A(DFI≤15%),group B(15%<DFI≤30%),and group C(DFI>30%).The process of in vitro fertilization embryo transfer of pairs in the three groups was observed,and their embryos were cultured.Results:There were no significant different in the abstinence time,semen volume,sperm concentration,normal fertilization rate,cleavage rate,biochemical pregnancy rate,and clinical pregnancy rate among the three groups(P>0.05).The forward sperm viability,normal sperm morphology rate,high-quality embryo rate,high-quality embryo sac rate,blastocyst formation rate,biochemical pregnancy rate,and clinical pregnancy rate of group C were significant lower than those of group A and B(P<0.05),but which had no significant different between group A and B(P>0.05).Conclusion:The increasing of DNA fragment index of spermatozoa will reduce of sperm forward motility and semen quality,so as to decrease the rate of high-quality embryo and embryo sac,blastocyst formation,which will lead to adverse pregnancy outcomes.

关 键 词：体外受精-胚胎移植 精液质量 精子DNA碎片指数 胚胎发育 妊娠结局 

分 类 号：R714.8[医药卫生—妇产科学]