基于视觉恐惧反应的行为分析  

Behavior Analysis of Fear Emotion

作　　者：戎晓媛 张玉楼 黄康[1,2] 刘楠 蔚鹏飞[1] RONG Xiaoyuan;ZHANG Yulou;HUANG Kang;LIU Nan;WEI Pengfei(Shenzhen Institutes of Advanced Technology,Chinese Academy of Sciences,Shenzhen 518055,China;Unversity of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]中国科学院深圳先进技术研究院,深圳518055 [2]中国科学院大学,北京100049

出　　处：《集成技术》2020年第4期36-46,共11页Journal of Integration Technology

基　　金：国家自然科学基金青年基金项目(31500861);深圳市基础研究学科布局项目(JCYJ20170411140807570)。

摘　　要：对于生物个体的生存和繁衍来说,采取合适的防御行为及时应对不同危险刺激的能力至关重要。模拟捕食者从上方快速逼近(Looming)可诱发小鼠产生冻结(Freezing)或逃跑(Flight)的本能防御反应。在传统的动物行为学研究中,通常采取耗时耗力的手动调节方式来给予危险刺激,限制了动物行为学研究效率的进一步提高。该研究运用光遗传技术及动物行为学对小鼠防御机制进行测试。具体地,利用新研发的反馈式小鼠行为分析系统,对小鼠面对同一类型但不同强度的危险刺激(如灰度值大小、光刺激频率高低)时的防御反应进行研究。结果显示,在较低灰度值的视觉刺激或低频率的光刺激条件下,小鼠倾向于原地冻结;在较高灰度值的视觉刺激或高频率的光刺激条件下,小鼠趋向于逃跑至安全区域。该反馈式小鼠行为分析系统的研发实现了在线实时分析小鼠行为和对小鼠的随机刺激,提高了实验效率和准确性。The ability of animals to respond to different life-threatening stimuli and take the most adaptive defensive behavior is essential for survival and breeding.Studies have found that a gradually expanding black disk in the upper field which simulates the looming of natural predators approaching from above can induce different instinct defensive behaviors of mice,including freezing or flight.In conventional animal behavior studies,time and labor-consuming manual operations are used to generate the stimuli,limiting the efficiency of animal behavior studies.In this paper,we have developed a closed-loop animal behavior regulation and analysis system.Based on this system,we have studied the defensive behavior of mice facing different stimuli.Looming with low gray value or low-frequency light stimulation would induce the freezing behavior of mice,which tend to escape to a safe area under looming with high gray value or high-frequency light stimulation.This study has demonstrated an automatic behavior regulation system capable of delivering random stimuli,which can further improve the efficiency and accuracy of the experiment.

关 键 词：恐惧反应 防御行为 随机刺激 

分 类 号：Q189[生物学—神经生物学]