响应面法优化乌饭树叶总黄酮超声提取工艺的研究  

Optimization of ultrasonic-assisted extraction process of total flavonoids from leaves of Vaccinium bracteatum Thunb.

作　　者：周鹏[1] 赵青[2] 黄婧[1] 张敏[1] Zhou Peng;Zhao Qing;Huang Jing;Zhang Min(Jiangsu Academy of Forestry, Nanjing 211153,China;Nanjing Forestry University, Nanjing 210037,China)

机构地区：[1]江苏省林业科学研究院,江苏南京211153 [2]南京林业大学,江苏南京210037

出　　处：《江苏林业科技》2020年第3期1-6,共6页Journal of Jiangsu Forestry Science & Technology

基　　金：江苏省林业科学研究院青年基金项目“LED光质对乌饭树组培苗生长及叶片黄酮积累的调控研究”(JAF-2016-04);中央财政林业科技推广示范资金项目“乌饭树良种繁育及栽培技术推广”(苏[2019]TG02)。

摘　　要：为确定乌饭树叶总黄酮超声辅助提取最佳工艺条件,以总黄酮提取量为指标,在单因素试验基础上,利用响应面法建立数学模型考察乙醇体积分数、液料比、超声时间及超声温度对乌饭树叶总黄酮提取量的影响,优化乌饭树叶总黄酮提取工艺。结果表明,最佳提取工艺条件为:以体积分数75%乙醇为提取溶剂,液料比65∶1 mL/g,超声时间80 min,超声温度65℃,在此工艺条件下的总黄酮提取量为23.41 mg/g。该结果与模型预测值相近,表明利用响应面法优化乌饭树叶总黄酮提取工艺合理可行。In order to determine the optimum conditions in the ultrasonic extraction of total flavonoids in Vaccinium bracteatum Thunb.leaves,response surface method was used to optimize the main technological parameters and get the regression model using total flavonoids extraction content as index,based on the results of single factor trial.The screened optimum technological conditions were as follows,75%of the ethanol concentration as the extraction solvent,liquid-solid ratio of 65 mL∶1 g,ultrasonic duration time of 80 min,ultrasonic temperature of 65℃.The confirmatory experiment value of the total flavonoids extraction content was 23.41 mg/g,so close to the predicted value of the regression model and the regression equation fitted well with the actual situation.The technological conditions could be referred as flavonoids extractionin in V.bracteatum Thunb.leaves.

关 键 词：乌饭树 总黄酮 提取 工艺 响应面法 

分 类 号：R284.2[医药卫生—中药学] S793.9[医药卫生—中医学]