模拟酸雨胁迫对夏枯草抗氧化酶活性和光合参数的影响  

Antioxidant Capacity and Photosynthetic Characteristics of Prunella vulgaris Seedlings in Response to Simulated Acid Rain Stress

作　　者：张利霞[1] 郝国宝 常青山[2] 刘伟[1] 薛娴[1] 陈苏丹[2] 陈园梦 张帅文 杨小菁 ZHANG Li-xia;HAO Guo-bao;CHANG Qing-shan;LIU Wei;XUE Xian;CHEN Su-dan;CHEN Yuan-meng;ZHANG Shuai-wen;YANG Xiao-jing(College of Agriculture,Henan University of Science and Technology,Luoyang 471000,China;College of Forestry,Henan University of Science and Technology,Luoyang 471000,China)

机构地区：[1]河南科技大学农学院,河南洛阳471000 [2]河南科技大学林学院,河南洛阳471000

出　　处：《中国草地学报》2020年第4期56-61,共6页Chinese Journal of Grassland

基　　金：河南省自然科学基金项目(182300410082);河南省科技攻关计划项目(182102110401);河南科技大学培育基金项目(4024-13350073);河南科技大学大学生研究训练计划(SRTP)项目(2019422)资助。

摘　　要：以夏枯草为材料,采用营养液模拟酸胁迫环境,研究不同梯度pH营养液(pH 4.5、4.0、3.5、3.0)对夏枯草抗氧化能力、叶绿素含量及光合能力的影响。结果表明:随着营养液pH值降低,夏枯草叶片过氧化物酶和抗坏血酸过氧化物酶活性、丙二醛含量和相对电导率均呈现上升趋势;超氧化物歧化酶活性、可溶性蛋白含量、水分利用效率和气孔限制值均呈现先上升后下降趋势;叶绿素、类胡萝卜素含量、净光合速率、气孔导度、蒸腾速率、过氧化氢酶活性均呈现下降趋势;胞间CO2浓度呈现先下降后上升的趋势。在pH值为3.5~4.5范围内,夏枯草净光合速率下降是由气孔因素引起;在pH 3.0处理下,夏枯草净光合速率显著下降是由非气孔因素引起。由此可见,夏枯草可以通过提高部分抗氧化酶活性来抵御酸胁迫伤害。To study the effect of simulated acid rain stress on the antioxidant, chlorophyll content and photosynthetic characteristics of Prunella vulgaris, different gradient pH nutrient solution(pH 4.5, 4.0, 3.5, 3.0) was used in this study. The results showed that activities of peroxidase(POD) and ascorbate peroxidase(APX), malondialdehyde(MDA) content and relative electrical conductivity of P. vulgaris were increased with the reducing of pH value, while the superoxide dismutase(SOD) activity, soluble protein content, water use efficiency(WUE) and stomatal limit value(Ls) were increased firstly and decreased subsequently. The acid rain stress significantly induced the reduction of chlorophyll and carotenoid contents, net photosynthetic rate(Pn), stomatal conductance(Gs), transpiration rate(Tr) and catalase(CAT) activity. Moreover, intercellular CO2 concentration(Ci) was decreased firstly and increased subsequently under the acid rain stress. The decrease of Pn in P. vulgaris leaves was caused by stomatal factors within pH 3.5~4.5. However, the decrease of Pn in P. vulgaris leaves was caused by non-stomatal factors when pH value was lower than 3.0. It can be concluded that P. vulgaris could prevent from acid rain damage by increasing certain antioxidant enzyme activities.

关 键 词：夏枯草 PH值 酸雨胁迫 光合作用 抗氧化酶 

分 类 号：S567.239[农业科学—中草药栽培]