NaCl胁迫下多年生黑麦草的代谢组学分析  

Metabonomics Analysis of Lolium perenne under NaCl Stress

作　　者：张彩峡 吴洪新[3] 毕玉芬[1] ZHANG Cai-xia;WU Hong-xin;BI Yu-fen(College of Animal Science and Technology,Yunnan Agricultural University,Kunming 650100,China;Tibet Vocational Technical College,Lhasa 850000,China;Institute of Grassland Research of CAAS,Hohhot 010010,China)

机构地区：[1]云南农业大学动物科技学院,云南昆明650100 [2]西藏职业技术学院,西藏拉萨850000 [3]中国农业科学院草原研究所,内蒙古呼和浩特010010

出　　处：《中国草地学报》2020年第4期62-72,共11页Chinese Journal of Grassland

基　　金：云南省重点研发计划项目“高寒山区饲草种植、收获、加工与利用”(2018BB001-03);西藏自治区高等学校科研创新团队建设项目(西藏职业技术学院2014-2017)。

摘　　要：利用基于气相色谱质谱联用技术的代谢组学方法,研究0、50mmol/L、100mmol/L、200mmol/L、300mmol/L、400mmol/L NaCl胁迫下多年生黑麦草代谢组分的变化,共检出391个色谱峰,利用Mann-Whitney和Kruskal-Wallis检验获得114种显著变化代谢物(P<0.01),采用s-plot分析获得17个靶标代谢物,用MetaboAnalyst4.0对17种代谢靶标进行通路富集分析获得8条显著变化的代谢通路(P<0.05),分别是淀粉和蔗糖代谢、甘氨酸丝氨酸和苏氨酸代谢、丙氨酸天冬氨酸谷氨酸代谢、氨酰tRNA生物合成、半乳糖代谢、乙醛酸和二羧酸代谢、氰基氨基酸代谢、光合碳循环。随着NaCl浓度升高,脯氨酸含量升高,抗坏血酸和水杨酸含量随之升高然后到400mmol/L浓度下降,吲哚乙酸在50mmol/L NaCl胁迫时升高,之后下降,乙烯生物合成前体物质1-氨基环丙烷基-1-羧酸则一直呈累积趋势。相对含水量和丙二醛含量在50mmol/L NaCl胁迫下分别升高和下降,而100~400mmol/L NaCl胁迫处理相对含水量逐渐下降,丙二醛含量逐渐升高。To clarify the response mechanism of perennial ryegrass(Lolium perenne) to salt stress, the changes of metabolic components of perennial ryegrass were analyzed by the GC/MS-based metabolomics method under NaCl stress(0, 50 mmol/L, 100 mmol/L, 200 mmol/L, 300 mmol/L and 400 mmol/L). A total of 391 chromatographic peaks were detected, and 114 metabolites with significant changes were obtained by Mann Whitney and Kruskal Wallis tests(P<0.01), furthermore, there were 17 target metabolites were obtained by s-plot analysis, and eight significant metabolic pathways were obtained by MetaboAnalyst 4.0, which were starch and sucrose metabolism;glycine, serine and threonine metabolism;alanine, aspartic acid and glutamic acid metabolism;aminoacyl tRNA biosynthesis;galactose metabolism;glyoxalic and dicarboxylic acid metabolism;cyano amino acid metabolism;photosynthetic carbon cycle. With the increase of NaCl concentration, proline content increased, ascorbic acid and salicylic acid content increased, and then decreased under 400 mmol/L NaCl stress. Indole-3-acetic acid increased under 50 mmol/L NaCl stress, and then decreased from 100 mmol/L to 400 mmol/L NaCl stress. 1-Aminocyclopropane-1-carboxylic acid, the precursor of ethylene biosynthesis, showed a cumulative trend. The relative water content(RWC) and MDA content increased and decreased respectively under 50 mmol/L NaCl stress, while RWC decreased and MDA increased gradually under 100 mmol/L to 400 mmol/L NaCl stress treatment.

关 键 词：多年生黑麦草 NACL胁迫 代谢组学 

分 类 号：S543.6[农业科学—作物学]