一株尿液标本分离获得Actinotignum schaalii的鉴定及生物学特性分析  

Identification and biological characteristics of Actinotignum schaalii isolated from urine

作　　者：李莎 杜晶辉 商希鹏 刘旭 李瑞仕[2] 蒋建春 LI Sha;DU Jing-hui;SHANG Xi-peng;LIU Xu;LI Rui-shi;JIANG Jian-chun(Clinical Laboratory,the First Teaching Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin 300380,China;Department of Pediatrics,the First Teaching Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin 300380,China)

机构地区：[1]天津中医药大学第一附属医院检验科,300380 [2]天津中医药大学第一附属医院儿科,300380

出　　处：《天津医药》2020年第8期760-763,共4页Tianjin Medical Journal

摘　　要：目的探讨Actinotignum schaalii的鉴定方法及生物学特性,为临床诊断Actinotignum schaalii引起的泌尿系感染提供依据。方法收集1例于2020年1月在天津中医药大学第一附属医院住院泌尿系感染患者的尿液标本,对其进行细菌培养及革兰染色,观察细菌形态特点。用基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)及16S rRNA基因测序技术进行鉴定。所得序列与GenBank数据库中的序列进行比对,并进行系统发育分析。结果该病例由Actinotignum schaalii引起泌尿系感染,为国内首例报道。经MALDI-TOF MS与16S rRNA基因检测鉴定为Actinotignum schaalii,属于放线棒菌属,为兼性厌氧、革兰阳性杆菌。判断其为引起泌尿系感染的新型致病菌。结论Actinotignum schaalii可能是引起泌尿系感染的新型致病菌。Objective To investigate the methods for identifying Actinotignum schaalii and to analyze its biological characteristics in order to provide a basis for the diagnosis of urinary tract infections.Methods The urine sample of a patient with urinary tract infection hospitalized in the First Teaching Hospital of Tianjin University of Traditional Chinese Medicine in January of 2020 was collected.Bacterial culture and Gram staining were used to observe the morphological characteristics.Matrix-assisted laser desorption/ionization-time of flight mass spectrometry(MALDI-TOF MS)and 16S rRNA sequencing were used for the identification.The obtained sequences were compared with those in GenBank database for phylogenetic analysis.Results The case was the first reported in China for a urinary tract infection caused by Actinotignum schaalii.MALDI-TOF MS and 16S rRNA sequencing identified the strain as Actinotignum schaalii,belonging to the genera Actinotignum,which was facultative anaerobic and Gram-positive bacterium.It was considered as a new type of pathogenic bacteria causing urinary tract infection.Conclusion Actinotignum schaalii may be a new pathogen that causes urinary tract infection.

关 键 词：放线菌属 泌尿道感染 光谱法 质量 基质辅助激光解吸电离 RNA 核糖体 16S 序列分析 RNA Actinotignum schaalii 

分 类 号：R378.994[医药卫生—病原生物学]