脐带间充质干细胞移植对急性颅脑创伤大鼠超氧化物歧化酶及白细胞介素-12的影响  

Effects of Umbilical Cord Mesenchymal Stem Cells Transplantation on Superoxide Dismutase and Interleukin-12 in Rats with Acute Traumatic Brain Injury

作　　者：彭定伟 张博 张秀娟 回月彤 王磊[6] 商崇智 王强 董化江[5] 徐健[5] 张赛 PENG Dingwei;ZHANG Bo;ZHANG Xiujuan;HUI Yuetong;WANG Lei;SHANG Chongzhi;WANG Qiang;DONG Huajiang;XU Jian;ZHANG Sai(Graduate School of Tianjin Medical University,Tianjin 300070,China;Department of Neurosurgery,Characteristic Medical Center of Chinese People′s Armed Police Forces,Tianjin 300162,China;Tianjin Neural Restoration Key Laboratory,Tianjin 300162,China;Dongxin Community Health Service Center of Tianjin Hedong District,Tianjin 300162,China;Logistics University of Chinese People′s Armed Police Forces,Tianjin 300189,China;Institute of Biomedical Engineering,Chinese Academy of Medical Sciences&Peking Union Medical College,Tianjin 300192,China)

机构地区：[1]天津医科大学研究生院,天津300070 [2]武警特色医学中心神经外科,天津300162 [3]天津市神经修复重点实验室,天津300162 [4]天津市河东区东新街社区卫生服务中心,天津300162 [5]武警后勤学院,天津300189 [6]中国医学科学院北京协和医学院生物医学工程研究所,天津300192

出　　处：《医学综述》2020年第15期3062-3066,共5页Medical Recapitulate

基　　金：国家自然科学基金(81801240)。

摘　　要：目的探讨人脐带间充质干细胞(UC-MSCs)对急性颅脑创伤(TBI)大鼠受损脑组织超氧化物歧化酶(SOD)和白细胞介素-12(IL-12)的影响。方法将45只Sprague-Dawley大鼠依据随机数字法分为对照组、TBI模型组和TBI干细胞治疗组,每组15只。采用免疫组织化学法、反转录聚合酶链反应、蛋白印迹法及酶联免疫吸附试剂盒等检测各组受损脑组织区域SOD和IL-12(含血清浓度)的表达情况,并比较各组脑组织IL-12信使RNA(mRNA)、SOD mRNA、SOD蛋白、IL-12蛋白的表达和SOD阳性细胞数以及血清IL-12水平。结果与对照组相比,TBI模型组脑组织IL-12 mRNA、SOD mRNA、IL-12蛋白和SOD蛋白表达水平均明显上调[(0.689±0.093)比(0.430±0.151)、(0.414±0.092)比(0.332±0.114)、(0.733±0.091)比(0.364±0.106)、(0.494±0.063)比(0.402±0.101)](P<0.05);TBI干细胞治疗组SOD mRNA和SOD蛋白表达水平明显上调[(0.630±0.163)比(0.332±0.114)、(0.774±0.103)比(0.402±0.101)](P<0.05),IL-12 mRNA和IL-12蛋白表达水平比较差异无统计学意义(P>0.05)。与TBI模型组相比,TBI干细胞治疗组IL-12 mRNA和IL-12蛋白表达水平明显下调[(0.386±0.104)比(0.689±0.093)、(0.413±0.164)比(0.733±0.091)](P<0.05),SOD mRNA和SOD蛋白表达水平明显上调(P<0.05)。对照组、TBI模型组、TBI干细胞治疗组大鼠SOD阳性细胞数分别为(0.031±0.007)、(0.069±0.006)、(0.123±0.013),TBI模型组和TBI干细胞治疗组大鼠SOD阳性细胞数明显多于对照组(P<0.01),TBI干细胞治疗组明显多于TBI模型组(P<0.01)。对照组、TBI模型组、TBI干细胞治疗组大鼠血清IL-12水平分别为(0.378±0.045)pg/mL、(5.450±0.941)pg/mL、(1.467±0.321)pg/mL,TBI模型组血清IL-12水平高于对照组(P<0.05),TBI干细胞治疗组低于TBI模型组(P<0.01)。结论UC-MSCs移植可提高TBI大鼠脑损伤区域SOD的表达,并抑制氧化应激反应,降低IL-12表达水平,有效控制炎症级联反应,有利于受损脑组织的恢复。Objective To investigate the effect of human umbilical cord mesenchymal stem cells(UC-MSCs)on superoxide dismutase(SOD)and interleukin-12(IL-12)in brain tissue of rats with acute traumatic brain injury(TBI).Methods A total of 45 Sprague-Dawley rats were randomly divided into a control group,a TBI model group and a TBI stem cell treatment group according to random number method,15 rats in each group.Immunohistochemistry,reverse transcription polymerase chain reaction,Western blotting and ELISA were used to detect the expression of SOD and IL-12(including serum concentration)near the damaged brain tissue in each group,and the expression of IL-12 messenger RNA(mRNA),SOD mRNA,SOD protein,IL-12 protein,the number of SOD positive cells and the level of serum IL-12 in each group were compared.Results Compared with the control group,the expression levels of IL-12 mRNA,SOD mRNA,IL-12 protein and SOD protein of the TBI model group were significantly up-regulated(0.689±0.093 vs 0.430±0.151,0.414±0.092 vs 0.332±0.114,0.733±0.091 vs 0.364±0.106,0.494±0.063 vs 0.402±0.101)(P<0.05);the expression levels of SOD mRNA and SOD protein of the TBI stem cell treatment group were significantly up-regulated(0.630±0.163 vs 0.332±0.114,0.774±0.103 vs 0.402±0.101)(P<0.05),while there was no statistically significant difference in the expression levels of IL-12 mRNA and IL-12 protein(P>0.05).Compared with the TBI model group,the expression levels of IL-12 mRNA and IL-12 protein in the TBI stem cell treatment group were significantly down-regulated(0.386±0.104 vs 0.689±0.093,0.413±0.164 vs 0.733±0.091)(P<0.05),while the levels of SOD mRNA and SOD protein were significantly up-regulated(P<0.05).The number of SOD positive cells in the control group,TBI model group and TBI stem cell treatment group was 0.031±0.007,0.069±0.006 and 0.123±0.013,respectively.The number of SOD positive cells in the TBI model group and TBI stem cell treatment group was significantly higher than that in the control group(P<0.01),and that in the TBI

关 键 词：颅脑创伤 脐带间充质干细胞 超氧化物歧化酶 白细胞介素-12 

分 类 号：R651.15[医药卫生—外科学]