微小RNA-107对非小细胞肺癌基因转化生长因子β受体2的调控作用  

Regulatory effect of microRNA-107 on transforming growth factor beta receptor 2 in non small cell lung cancer

作　　者：江强[1] 周圣瑜 许玉华[1] JIANG Qiang;ZHOU Sheng-yu;XU Yu-hua(Department of Cardiology,Jiangxi Thoracic Hosital,Nanchang 330000,Jiangxi Province,China)

机构地区：[1]江西省胸科医院心内科,江西南昌330006

出　　处：《中国临床药理学杂志》2020年第13期1842-1844,1849,共4页The Chinese Journal of Clinical Pharmacology

摘　　要：目的研究微小核糖核酸(miRNANA)-107对非小细胞肺癌(NSCLC)基因转化生长因子β受体Ⅱ(TGFβR2)的调控作用。方法将对数生长期A549细胞随机分为4组:空白对照组(空白试剂)、第1转染组、第2转染组和第3转染组。按照脂质体2000说明方法,这3组分别转染miRNA-170阴性对照物、miRNA-170拟似物和miRNA-170阻遏物。用实时荧光定量-PCR法检测细胞miRNA-107和TGFβR2基因表达,用四甲基偶氮唑蓝法检测细胞增殖活性(OD值),用流式细胞仪分析细胞凋亡率,用划痕试验检测细胞迁移力,用Transwell小室法检测细胞侵袭能力。结果空白对照组、第1转染组、第2转染组和第3转染组的miRNA-170基因相对表达量分别为0.98±0.08,0.93±0.09,3.81±0.27和0.24±0.05;上述4组TGFβR2基因的相对表达量分别为0.90±0.09,0.91±0.07,0.39±0.08和3.00±0.11;上述4组72 h增殖活性分别为0.76±0.10,0.77±0.09,0.26±0.09和1.27±0.09;上述4组细胞的凋亡率分别为(8.24±0.93)%,(8.09±0.89)%,(29.85±6.73)%和(3.88±0.99)%;上述4组的细胞划痕愈合率分别为(74.03±8.14)%,(73.32±7.93)%,(32.47±6.36)%和(91.24±5.03)%;上述4组的穿膜细胞数分别为(72±9),(73±10),(28±9)和(134±14)个细胞。上述指标:第2转染组与空白对照组和第1转染组比较,差异均有统计学意义(均P<0.05);第3转染组和第2转染组比较,差异均有统计学意义(均P<0.05)。结论miRNA-107通过靶向TGFβR2抑制NSCLC细胞增殖、迁移、侵袭和细胞周期,miRNA-107可能是NSCLC潜在的治疗靶点。Objective To investigate the regulatory effect of microRNA-107(miRNANA-107)on transforming growth factor beta receptor 2(TGFβR2)in non small cell lung cancer(NSCLC).Methods A549 cells were randomly divided into four groups:blank control group(blank reagent),first transfection group,second transfection group and third transfection group.The miRNA-170 negative control,MIRNA-170 mimic and miRNA-170 repressor in the three transfection groups were transfected respectively according to the instructions of Lipofectamine 2000.Expression of miRNA-107 and TGFβR2 mRNA was detected by quantitative real-time PCR.Cell proliferation activity(OD value)was detected by measurement of trititaed thymidine.Apoptosis rate was analyzed by flow cytometry.Cell migration was detected by scratch test and cell invasion was detected by Transwell cell method.Results Relative expression of miRNA-170 mRNA in blank control group,first transfection group,second transfection group and third transfection group were 0.98±0.08,0.93±0.09,3.81±0.27 and 0.24±0.05,respectively;relative expression of TGFβR2 mRNA in above four groups were 0.90±0.09,0.91±0.07,0.39±0.08 and 3.00±0.11,respectively;Cell proliferation activity in above four groups were 0.76±0.10,0.77±0.09,0.26±0.09 and 1.27±0.09,respectively;apoptosis rates in above four groups were(8.24±0.93)%,(8.09±0.89)%,(29.85±6.73)%and(3.88±0.99)%,respectively;scratch healing rates in above four groups were(74.03±8.14)%,(73.32±7.93)%,(32.47±6.36)%and(91.24±5.03)%,respectively;number of transmembrane cells in above four groups were(72±9),(73±10),(28±9)and(134±14)cells,respectively.Comparison between second transfection group and blank control group,first transfection group,the difference of the factors were significant(all P<0.05).Comparison between third transfection group and second transfection group,the difference of the factors were significant(all P<0.05).Conclusion miRNA-107 can inhibit NSCLC cell proliferation,migration,invasion and cell cycle by targeting TGFβR2,which ma

关 键 词：非小细胞肺癌 生长因子β受体2 微小RNA A549细胞 细胞增殖 

分 类 号：R97[医药卫生—药品]