和厚朴酚对高糖诱导的足细胞氧化应激及凋亡的影响  

Effects of honokiol on oxidative stress and apoptosis induced by high glucose in podocytes

作　　者：彭君[1] 彭家清[1] 秦鹏[1] 罗先荣 张敏[1] PENG Jun;PENG Jia-qing;QIN Peng;LUO Xian-rong;ZHANG Min(Department of Nephrology^Jingzhou Central Hospital,Jingzhou 434020,Hubei Province,China)

机构地区：[1]湖北省荆州市中心医院肾内科,湖北荆州434020

出　　处：《中国临床药理学杂志》2020年第13期1870-1874,共5页The Chinese Journal of Clinical Pharmacology

基　　金：荆州市科技发展计划基金资助项目(2015049)。

摘　　要：目的探讨和厚朴酚对高糖诱导的足细胞氧化应激、凋亡的影响及其机制。方法实验分为对照组(常规培养)、高糖组(30 mmol·L^-1 D-葡萄糖)、低剂量实验组(低剂量和厚朴酚)、中剂量实验组(中剂量和厚朴酚)、高剂量实验组(高剂量和厚朴酚)、第1转染组(30 mmol·L^-1的D-葡萄糖+miR-182-5p阳性对照质粒)、第2转染组(30 mmol·L^-1的D-葡萄糖+miR-182-5p过表达质粒)、第3转染组(30 mmol·L^-1的D-葡萄糖+NOX4阴性对照质粒)、第4转染组(30 mmol·L^-1的D-葡萄糖+NOX4抑制表达质粒)、第5转染组(10μmol·L^-1和厚朴酚+30 mmol·L^-1的D-葡萄糖+miR-182-5p阴性对照质粒)、第6转染组(10μmol·L^-1和厚朴酚+30 mmol·L^-1的D-葡萄糖+miR-182-5p抑制表达质粒)。用试剂盒检测丙二醛(malondialdehyde,MDA)、超氧化物歧化酶(superoxide dismutase,SOD)含量;流式细胞术检测细胞凋亡。结果对照组、高糖组、低剂量实验组、中剂量实验组、高剂量实验组、第1转染组、第2转染组、第3转染组、第4转染组、第5转染组、第6转染组细胞凋亡率分别为(6.28±0.64)%,(27.13±2.87)%,(20.16±2.07)%,(15.74±1.52)%,(11.24±1.12)%,(27.36±2.78)%,(13.56±1.32)%,(27.98±2.71)%,(14.02±1.38)%,(11.05±1.16)%,(20.45±2.08)%;MDA含量分别为(5.23±0.54),(19.21±1.28),(16.32±1.22),(12.37±1.45),(8.34±0.73),(19.87±1.88),(9.47±0.95),(21.48±2.16),(12.42±1.34),(8.03±0.79)和(17.65±1.73)nmol·mg^-1;SOD活性分别为(97.25±7.37),(31.42±3.28),(49.32±4.87),(62.47±6.58),(83.46±6.11),(32.49±3.17),(79.35±7.83),(34.76±3.49),(75.43±7.28),(85.34±8.29)和(43.26±5.07)U·mg^-1。高糖组与对照组比较,低剂量实验组、中剂量实验组、高剂量实验组与高糖组比较,第2转染组与第1转染组比较,第4转染组与第3转染组比较,第6转染组与第5转染组比较,差异均有统计学意义(均P<0.05)。结论和厚朴酚对高糖诱导的肾足细胞MPC5凋亡有保护作用,可减轻氧化应激,其机制与上调miR-18Objective To investigate the effect of honokiol on high glucose-induced podocyte oxidative stress and apoptosis and its mechanism.Methods The experiment was divided into control group(routine treatment),high-glucose group(30 mmol·L^-1 D-glucose),test-L group(low-dose honokiol),test-M group(medium dose honokiol),test-H group(high-dose honokiol),transfection-1 group(30 mmol·L^-1 D-glucose+miR-182-5 p positive control plasmid),transfection-2 group(30 mmol·L^-1 D-glucose+miR-182-5 p overexpression plasmid),transfection-3 group(30 mmol·L^-11 D-glucose+NOX4 negative control plasmid),transfection-4 group(30 mmol·L^-1 D-glucose+NOX4 inhibitory expression plasmid),transfection-5 group(10μmol·L^-1 honokiol+30 mmol·L^-1 D-glucose+miR-182-5 p negative control plasmid),transfection-6 group(10μmol·L^-1 honokiol+30 mmol·L^-1 D-glucose+miR-182-5 p inhibition expression plasmid).The kit was used to detect malondialdehyde(MDA)and superoxide dismutase(SOD)contents;flow cytometry was used to detect cel apoptosis.Results In the control group,high-glucose group,test-L,test-M,test-H group,transfection-1 group,transfection-2 group,transfection-3 group,transfection-4 group,transfection-5 group and transfection-6 group,the apoptotic rates were(6.28±0.64)%,(27.13±2.87)%,(20.16±2.07)%,(15.74±1.52)%,(11.24±1.12)%,(27.36±2.78)%,(13.56±1.32)%,(27.98±2.71)%,(14.02±1.38)%,(11.05±1.16)%,(20.45±2.08)%,respectively;the MDA contents were(5.23±0.54),(19.21±1.28),(16.32±1.22),(12.37±1.45),(8.34±0.73),(19.87±1.88),(9.47±0.95),(21.48±2.16),(12.42±1.34),(8.03±0.79)and(17.65±1.73)nmol·mg^-1,respectively;SOD activities were(97.25±7.37),(31.42±3.28),(49.32±4.87),(62.47±6.58),(83.46±6.11),(32.49±3.17),(79.35±7.83),(34.76±3.49),(75.43±7.28),(85.34±8.29)and(43.26±5.07)U·mg^-1,respectively.Comparison between high-glucose group and control group,comparison between the test-L,the test-M group,the test-H group and high-glucose group,comparison between the transfection-2 group and transfection-1 group,comparison between

关 键 词：和厚朴酚 足细胞 氧化应激 凋亡 

分 类 号：R28[医药卫生—中药学]