中药玄参中化学成分及其抗炎活性研究  

Chemical Constituents from the Roots of Scrophularia ningpoensis Hemsl and Their Anti-inflammatory Activities

作　　者：霍玉峰 詹志来 王怀立[1] 魏二虎[1] 贾沛生[1] 刘会琼[1] 范晓蕾[1] 闫坤丽 HUO Yu-feng;ZHAN Zhi-lai;WANG Huai-li;WEI Er-hu;JIA Pei-sheng;LIU Hui-qiong;FAN Xiao-lei;YAN Kun-li(Department of Pediatrics,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China;National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]郑州大学第一附属医院小儿内科,郑州450052 [2]中国中医科学院中药资源中心,北京100700

出　　处：《中国药学杂志》2020年第14期1207-1214,共8页Chinese Pharmaceutical Journal

基　　金：郑州大学第一附属医院院内青年创新基金项目资助。

摘　　要：目的研究玄参(Scrophularia ningpoensis Hemsl)的化学成分。方法通过各种柱色谱进行分离纯化,根据理化性质和谱学数据进行结构鉴定。并利用脂多糖(LPS)诱导的BV2细胞模型对分离得到的化合物进行抗炎活性筛选。结果分离并鉴定了18个化合物:2″′-乙酰安格洛苷C(1),囊状毛蕊花苷(2),6-O-α-L-(2″-O-阿魏酰基)吡喃鼠李糖基梓醇(3),玄参苷(4),哈平卧钩果草别苷A(5),6″-O-β-D-吡喃葡萄糖基哈巴俄苷(6),哈巴俄苷(7),8-O-(p-香豆酰基)-哈巴苷(8),8-O-阿魏酰哈巴苷(9),6-O-α-D-吡喃半乳糖基哈巴俄苷(10),6′-O-桂皮酰基哈巴苷(11),安哥罗苷B(12),安哥罗苷C(13),玄参苷B1(14),2-(3-羟基-4-甲氧基苯基)乙基-O-α-L-吡喃阿拉伯糖基-(1→6)-O-α-L-吡喃鼠李糖基-(1→3)]-β-D-吡喃葡萄糖苷(15),达伦代黄芩苷B(16),6-O-咖啡酰基-β-D-呋喃果糖基-(2→1)-α-D-吡喃葡萄糖苷(17)和斩龙剑苷A(18)。结论化合物1为一个新化合物,化合物2~4,12,14和17为首次从玄参中分离得到。化合物1,12和13对LPS诱导BV2细胞中NO的释放具有抑制活性。OBJECTIVE To study the chemical constituents in the roots of Scrophularia ningpoensis Hemsl.METHODSE Compounds were isolated by chromatographic techniques and their structures were elucidated by spectral methods.The anti-inflammatory activities of all isolated compounds were evaluated by lipopolysaccharides(LPS)-stimulated BV2 cells model.RESULTS Eighteen compounds were isolated and identified as 2″′-acetyl angroside C(1),saccatoside(2),6-O-α-L-(2″-O-feruloyl)rhamnopyranosylcatalpol(3),scrophularioside(4),harprocumbide A(5),6″-O-β-D-glucopyranosylharpagoside(6),harpagoside(7),8-O-(p-coumaroyl)-harpagide(8),8-O-feruloylharpagide(9),6-O-α-D-galactopyranosylharpagoside(10),6′-O-cinnamoylharpagide(11),angoroside B(12),angoroside C(13),scrophuloside B1(14),2-(3-hydroxy-4-methoxyphenyl)ethyl-O-α-L-arabinopyranosyl-(1→6)-O-α-L-rhamnopyranosyl-(1→3)]-β-D-glucopyranoside(15),darendoside B(16),6-O-caffeoyl-β-D-fructofuranosyl-(2→1)-α-D-glucopyranoside(17),and sibirioside A(18).CONCLUSION Compound 1 is new compound,and compounds 2-4,12,14,and 17 are isolated from this plant for the first time.Compounds 1,12,and 13 show significant inhibition against nitric oxide production in LPS-stimulated BV2 cells model.

关 键 词：玄参 环烯醚萜 苯乙醇苷 苯丙素 抗炎活性 

分 类 号：R932[医药卫生—生药学]