微流体液相色谱质谱联用分析头发中11种苯二氮卓类安眠镇静药物  

Ultrasensitive Analysis of 11 Benzodiazepines in Hair Samples Using Microfluidic Technology plus LC-MS/MS

作　　者：蒋文慧[1] 王朝虹 赵蒙 李虹 褚建新 JIANG Wenhui;WANG Zhaohong;ZHAO Meng;LI Hong;CHU Jianxin(The People’s Procuratorate of Hangzhou,Hangzhou 310014,China;Procuratorial Technical Information Center of The Supreme People’s Procuratorate of China,Beijing 100144,China;Ministry of Public Security’Key Laboratory of Narcotics Assay and Control Technology,Kunming 650500,China;The People’s Procuratorate of Zhejiang Province,Hangzhou 310012,China)

机构地区：[1]杭州市人民检察院,杭州310014 [2]最高人民检察院检察技术信息研究中心,北京100144 [3]毒品分析及禁毒技术公安部重点实验室,昆明650500 [4]浙江省人民检察院,杭州310012

出　　处：《刑事技术》2020年第4期358-364,I0001-I0003,共10页Forensic Science and Technology

基　　金：公安部科技强警基础工作专项项目(2016GABJC50)。

摘　　要：目的建立新的微流体液相色谱质谱联用法分析头发中11种苯二氮卓类安眠镇静药物(劳拉西泮、氯硝西泮、溴西泮、氟硝西泮、阿普唑仑、替马西泮、氯氮卓、奥沙西泮、地西泮、7-胺基氟硝西泮和硝西泮)。方法头发样品应用液液萃取法进行提取、微流控液相色谱质谱法进行检测。结果11种苯二氮卓类安眠镇静药物的线性回归系数均大于0.99,分析精密度和提取回收率分别为2%~16%和71.7%~114.9%。最小检测限和最小定量限分别为0.008~0.03pg/mg和0.025~0.125pg/mg。结论微流体液相色谱质谱联用法比传统的LC-MS/MS方法灵敏度高2~10倍。此方法已成功应用于实际毛发样本分析,可作为法医毒理学毛发中痕量成分检验方法。Objective To establish a novel micro fluidic LC-MS/MS method for analyzing 11 benzodiazepines(lorazepam,clonazepam,bromazepam,flunitrazepam,alprazolam,temazepam,chlordiazepam,oxazepam,diazepam,7-aminoflunitrazepam and nitrazepam)in hair samples.Methods A simple liquid-liquid extraction(LLE)procedure was applied for preparing the hair sample,which was afterwards undergone into the LC-MS/MS analysis.Results A good linearity for the target analytes was obtained with the regression coefficients above 0.99.Precisions and extraction efficiencies were in the range of 2.0%~16.0%and 71.7%~114.9%,respectively.LODs and LOQs of the analytes ranged from 0.008 to 0.03 pg/mg and 0.025 to 0.125 pg/mg,respectively.Conclusions The sensitivity of the microluidic LC-MS/MS method is of ten-fold higher than the traditional LC-MS/MS methods,leaving a successful application to analyze a real hair sample,therefore able to be an alternative approach for forensic toxicological analysis of trace drug testing in hair.

关 键 词：法医毒理学 微流体LC-MS/MS 苯二氮卓类 毛发 液液萃取 

分 类 号：DF795.1[医药卫生—法医学]