基于网络药理学的金莲花活性成分抗炎机制研究  

Anti-Inflammatory Mechanism of Active Components of Trollius Based on Network Pharmacology

作　　者：郭艳丽[1] 刘维丽[1] 鞠爱霞[1] 马伟[1] GUO Yanli;LIU Weili;JU Aixia;MA Wei(Heilongjiang University of Chinese Medicine,Harbin 150040,China)

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《中医药学报》2020年第8期25-28,共4页Acta Chinese Medicine and Pharmacology

基　　金：国家林业公益专项资助(201004079);黑龙江自然科学基金联合引导项目(LH2019H107)。

摘　　要：目的:利用网络药理学的研究方法,探索金莲花抗炎作用机制。方法:利用中药系统药理学数据库与分析平台搜索金莲花活性成分及活性成分相关靶点,在Uniprot数据库进行靶点蛋白的基因校正后,利用Cytoscape 3.7.2软件构建活性成分-靶点基因网络;在NCBI数据库,搜索"inflammation",得到与人类炎症相关的基因,用Cytoscape 3.7.2软件构建炎症靶点相互作用网络,将其与金莲花活性成分-靶点网络融合,获得金莲花活性成分的抗炎作用靶点。结果:中药系统药理学数据库与分析平台(TCMSP)检索到金莲花口服利用度≧30%,类药性≧0.18的活性成分7个,7个活性成分作用靶点共计183个蛋白,在Uniprot数据库检索并校正得到靶点基因166个;将6个活性成分与靶点基因数据导入Cytoscape 3.7.2软件,得到自由度≥5的靶点基因3个,分别是Ptgs1、Ptgs2、Ncoa2;在NCBI网站数据库检索"inflammation",得到与人类炎症相关基因1882个;利用Cytoscape 3.7.2软件将6个活性成分的166个靶点与炎症相关1882个靶点构建金莲花-抗炎的预测靶点,得到金莲花活性成分与抗炎靶点的交集共计87个(去除重复),分别是刺槐素抗炎靶点16个,β-谷甾醇抗炎靶点15个,谷甾醇抗炎靶点2个,果胶蛋白抗炎靶点6个,玄参黄酮抗炎靶点5个,槲皮素抗炎靶点80个。结论:槲皮素是金莲花抗炎作用靶点最多的活性物质;Ptgs1、Ptgs2是金莲花5种活性成分共有抗炎靶点;推测金莲花发挥抗炎作用的活性成分可能是槲皮素。Objective: To explore the anti-inflammatory mechanism of Trollius by using the method of network pharmacology. Methods: Using the pharmacology database and analysis platform of TCM system to search for the active components and related targets of active components of Trollius after gene correction of targets protein in Uniprot database. In the NCBI database, the genes related to human inflammation were obtained by searching for "inflammation", the anti-inflammatory targets of the active components of Trollius and the interaction network of inflammatory targets were constructed by using the software of Cytoscape 3.7.2. Results: In TCMSP, 7 active components with oral utilization≥30% and generic≥0.18 were found, A total of 183 proteins were targeted by 7 active components. 166 target genes were retrieved and corrected in Uniprot. The data of 6 active components and target genes were imported into the software of Cytoscape 3.7.2, and 3 target genes with degree of freedom≥5 were obtained, namely Ptgs1, Ptgs2 and Ncoa2. According to the NCBI, 1,882 genes related to human inflammation were obtained. The 166 targets of six active compounds and 1,882 targets related to inflammation were used to construct the prediction target of anti-inflammatory of Trollius by using the software of Cytoscape 3.7.2. The total number of intersection of the active components and anti-inflammatory targets of Trollius was 87, there were 16 anti-inflammatory targets of Robinia, 15 anti-inflammatory targets of β-sitosterol, 2 targets of sitosterol, 6 targets of pectin protein, 5 targets of Xuanshen flavone and 80 anti-inflammatory targets of quercetin. Conclusion: Quercetin is the most active anti-inflammatory target substance of Trollius. Ptgs1 and Ptgs2 are the common anti-inflammatory targets of five active components of Trollius. It is suggested that quercetin may be the active component with anti-inflammatory effect in Trollius.

关 键 词：金莲花 炎症 槲皮素 网络药理学 

分 类 号：R285[医药卫生—中药学]