基于网络药理学探讨吴茱萸-黄连药对治疗胃炎的机制研究  

Network Pharmacology-Based Study on Mechanisms of Drug Pair of Wuzhuyu-Huanglian in Treating Gastritis

作　　者：张慧[1] 王艳艳[1] 黄莉莉[1] 毛子玮 李廷利[1] ZHANG Hui;WANG Yanyan;HUANG Lili;MAO Ziwei;LI Tingli(Heilongjiang University of Chinese Medicine,Harbin 150040,China)

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《中医药学报》2020年第8期29-36,共8页Acta Chinese Medicine and Pharmacology

基　　金：国家自然科学基金项目(87214114);黑龙江省大学生创业实践项目(201910228084,201910228053)。

摘　　要：目的:本文通过应用生物信息学技术对吴茱萸-黄连药对进行网络药理学分析,探索吴茱萸-黄连药对治疗胃炎的潜在靶点及作用机制。方法:运用数据库TCMSP、Swiss Target Prediction、STITCH、GeneCard等检索吴茱萸-黄连药对活性成分、作用靶点以及胃炎相关靶点,应用数据库STRING分析各靶点之间的相互作用。利用Cytoscape3.7.2软件进行药物-成分-疾病-靶点网络分析,在此基础上运用数据库Bioconductor进行GO(gene ontology)过程分析,以及KEGG(Kyoto Encyclopedia of Genes and Genomes)通路富集分析。结果:在相关的数据库筛选之后,得到黄连的潜在活性成分8个,吴茱萸的潜在活性成分17个,二者相同活性成分有2种。吴茱萸-黄连药对筛选得到的靶点有79个,与胃炎靶点进行交集得到共有靶点基因是18个,如CASP3、VEGFA、EGFR、IL-6、MYC、CCND1等。吴茱萸-黄连药对治疗胃炎的主要功能是结合各种不同功能的蛋白如:蛋白磷酸酶、激活转录因子受体、细胞因子受体、凋亡受体、血管内皮生长因子受体等。涉及到的通路有HIF-1信号通路、肿瘤坏死因子信号通路,以及多种癌症通路和病毒感染通路。结论:吴茱萸-黄连药对在胃炎治疗过程中运用多通路联合协同方式,为胃炎的防治和治疗提供了可行性理论指导以及对今后的胃炎研究提供了新思路。Objective: To explore the potential targets and mechanism of the drug pair of Wuzhuyu-Huanglian(Euodlae Fructus-Coptidis Rhizoma) in the treatment of gastritis by the network pharmacology analysis and the application of bioinformatics technology. Methods: Database TCMSP, Swiss Target Prediction, STITCH and GeneCard were used to retrieve the active ingredients, targets and gastritis related targets of Wuzhuyu-Huanglian. The database STRING was applied to analyze the interaction between each target. The drug-component-disease-target network analysis was carried out by using Cytoscape 3.7.2 software. On this basis, the database Bioconductor was used for GO(gene ontology) process analysis and KEGG(Kyoto Encyclopedia of Genes and Genomes) pathway enrichment analysis. Results: After screening the relevant database, 8 potential active ingredients belong to Huanglian and 17 potential active ingredients of Wuzhuyu were obtained. 79 targets were screened from Wuzhuyu-Huanglian herbal pair, 18 target genes were obtained by intersecting with targets of gastritis disease, such as CASP3, VEGFA, EGFR, IL-6, MYC, CCND1 and so on. The main function of Wuzhuyu-Huanglian herbal pair in treating gastritis was to combine proteins with various functions, such as protein phosphatase, activated transcription factor receptor, cytokine receptor, apoptosis receptor, VEGF receptor and so on. The pathways involved were HIF-1 signaling pathway, tumor necrosis factor signaling pathway, and various cancer pathways and viral infection pathways. Conclusion: Wuzhuyu-Huanglian herbal pair provided a multi-channel and a synergistic approach in the treatment of gastritis, which supplied a feasible theoretical guidance for the prevention and treatment of gastritis and offered new ideas for future research on gastritis.

关 键 词：吴茱萸-黄连药对 胃炎 网络药理学 

分 类 号：R285[医药卫生—中药学]