三七多糖含量测定方法比较  

Comparison of Determination Methods of Polysaccharide Content in Panax Notoginseng

作　　者：蔡群虎 刘石磊 袁敏惠 王芬 胡会泽 张芳盛 郭霞 刘晓丽 辛文锋 CAI Qun-hu;LIU Shi-lei;YUAN Min-hui;WANG Fen;HU Hui-ze;ZHANG Fang-sheng;GUO Xia;LIU Xiao-li;XiIN Wen-feng(Yunnan Panax Notoginseng Science and Technology Co.,Ltd.,Kunming 650106,China;National and Local Joint Engineering Research Center of Panax Notoginseng Resource Protection and Utilization Technology Kunming 650106,China)

机构地区：[1]云南三七科技有限公司,云南昆明650106 [2]三七资源保护与利用技术国家地方联合工程研究中心,云南昆明650106

出　　处：《云南中医中药杂志》2020年第8期59-62,共4页Yunnan Journal of Traditional Chinese Medicine and Materia Medica

基　　金：国家科技部项目(2017YFC1702505)。

摘　　要：目的通过比较硫酸苯酚法和硫酸蒽酮法在测定三七多糖时的优劣性,建立更准确的三七多糖含量测定检测方法,为三七、三七剪口的多糖质量评价提供依据。方法依次用80%乙醇和纯水提取三七药材,水提部分得到三七多糖,对所得多糖采用硫酸-苯酚法和硫酸-蒽酮法分别测量三七粗多糖的含量。结果从精密度的角度来看,苯酚硫酸法较蒽酮硫酸法可靠(RSD=0.419<3.66)。从回收率的角度来看,使用苯酚硫酸法测定时,回收率更高(98.06>97.60)。结论苯酚硫酸法为更准确测定三七粗多糖含量的检测方法,此外,苯酚硫酸法还具有操作更简便、安全、快捷等优点。Objective:To establish a more accurate method for the determination and detection of notoginseng polysaccharide content by comparing the pros and cons of the sulfuric phenol method and the anthrone sulfate method in the determination of notoginseng polysaccharides and provide an evaluation basis for the quality of polysaccharides in notoginseng and notoginseng snip.Methods:The medicinal materials of Panax notoginseng were extracted with 80%ethanol and pure water in sequence,and the polysaccharides of Panax notoginseng were obtained by water extraction.The content of crude polysaccharides of Panax notoginseng was measured by the sulfuric acid-phenol method and the sulfuric acid-anthrone method.Results:From a precision point of view,the phenol sulfuric acid method was more reliable than the anthrone sulfuric acid method(RSD=0.419<3.66).From the perspective of recovery rate,the recovery rate is higher when the phenol sulfuric acid method was used for determination(98.06>97.60).Conclusion:The phenol-sulfuric acid method is a more accurate method to determine the content of the crude polysaccharides of Panax notoginseng.In addition,the phenol-sulfuric acid method is easier to operate,and safer and faster.

关 键 词：三七粗多糖 硫酸苯酚法 硫酸蒽酮法 含量测定 

分 类 号：R285[医药卫生—中药学]