印加孔雀草对土壤细菌群落多样性的影响  

The Effect of Tagetes minuta L.on the Diversity of Soil Bacterial Community

作　　者：郓玲玲 张瑞海[1] 宋振[1] 付卫东[1] 王然 王忠辉 张国良[1] YUN Lingling;ZHANG Ruihai;SONG Zhen;FU Weidong;WANG Ran;WANG Zhonghui;ZHANG Guoliang(Institute of Environment and Sustainable Development in Agriculture,Chinese Academy of Agricultural Sciences,Beijing 100081,China)

机构地区：[1]中国农业科学院农业环境与可持续发展研究所,北京100081

出　　处：《生态环境学报》2020年第5期901-909,共9页Ecology and Environmental Sciences

基　　金：国家自然科学基金面上项目(417977203);国家重点研发计划项目(2016YFC1201203)。

摘　　要：印加孔雀草(Tagetes minuta L.)入侵对生态系统和生物多样性造成危害,从土壤微生态学的角度探究印加孔雀草入侵对土壤细菌群落结构和多样性的影响,有助于深入揭示其入侵导致生态系统退化的机制。采用第二代高通量测序技术,以万寿菊(Tagetes erecta L.)为对照植物,设置不同植物的处理(裸土CK、万寿菊Te、印加孔雀草与万寿菊混种TmTe、印加孔雀草Tm),比较了各处理中土壤细菌群落结构的差异。结果表明:(1)TmTe的Ace指数显著低于CK、Tm且Chao指数显著低于CK,Tm的Shannon指数显著高于CK且Simpson指数显著低于CK,混种处理细菌群落丰富度最低,印加孔雀草单优群落处理群落多样性最高;(2)4个处理共有的OTUs为3169个,3个植物处理中,TmTe的细菌群落数最低,为3814个OTU,Tm细菌群落数最高,为4110个OTU;(3)相对丰度最高的前5个门分别是变形菌门(Proteobacteria)、酸杆菌门(Acidobacteria)、放线菌门(Actinobacteria)、绿弯菌门(Chloroflexi)和芽单胞菌门(Gemmatimonadetes),在各处理中所占比例之和皆为80%以上;(4)放线菌门、硝化螺旋菌门(Nitrospirae)、变形菌门、拟杆菌门(Bacteroidetes)、绿弯菌门以及芽单胞菌门与土壤全钾含量呈显著正相关(P<0.05)。蓝细菌门(Cyanobacteria)与环境因子之间的关系不同于其他菌门,相关性各有不同,但差异不显著。多囊粘细菌科(Polyangiaceae)、侏囊菌科(Nannocystaceae)、念珠藻科(Nostocaceae)等8个科的10个OTU类群共同存在于Tm vs CK和TmTe vs CK但不属于TmTe vs CK,表明其在印加孔雀草入侵过程中起着重要作用。该研究为揭示印加孔雀草入侵对土壤微生态影响机制提供了科学依据。The invasion of Tagetes minuta L.is harmful to ecosystem and biodiversity.The study of its impact on soil microbial community will help to reveal the mechanism of ecosystem degradation caused by its invasion.In this study,using T.erecta as the control plant,setting up different treatments,including soil without plants(CK),T.erecta dominated habitat(Te),T.erecta and T.minuta mixed habitat(TmTe),T.minuta dominated habitat(Tm),differences of soil bacterial community structure of T.minuta was compared through high-throughput sequencing.The results showed that:(1)The Ace index of TmTe was significantly lower than CK and Tm,the Chao index of TmTe was significantly lower than CK,the Shannon index of Tm was significantly higher than CK and the Simpson index of Tm was significantly lower than CK.The richness of bacterial community was the lowest in mixed treatment,and the diversity of T.minuta dominated treatment was the highest in all the treatments.(2)The total number of OTUs in four treatments was 3169,bacterial community number in TmTe was lowest,presenting 3814 OTUs,while bacterial community number in Tm was the highest,which was 4110.(3)The top five relative abundances of soil samples were Proteobacteria,Acidobacteria,Actinobacteria,Chloroflexi and Gemmatimonades,accounting for more than 80% in each treatment;And(4)actinobacteria,Nitrospirae,Proteobacteria,Bacteroidetes,Chloroflexi and Gemmatimonades were positively correlated with total potassium content in soil(P<0.05).The relationship between Cyanobacteria and environmental factors is different from other Phylum,but the difference is not significant.10 OTU groups of 8 families,including Polyangiaceae,Nannocystaceae and Nostocaceae,are coexisted in Tm vs CK and TmTe vs CK but not in TmTe vs CK,indicating that they play an important role in the invasion process of T.minuta.The results of this study provide theoretical basis for revealing the soil micro-ecological mechanism of T.minuta invasion.

关 键 词：外来入侵植物 印加孔雀草 高通量测序 土壤细菌 群落多样性 

分 类 号：S45[农业科学—植物保护] S154.3